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70954 Rosetta™(DE3) Competent Cells - Novagen

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70954
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          Description
          OverviewRosetta™ host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLysS, the rare tRNA genes are present on the same plasmids that carries the T7 lysozyme gene.

          DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
          Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)


          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number70954
          Brand Family Novagen®
          References
          Product Information
          Components
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          70954-3 07790788052904
          70954-4 07790788052911

          Documentation

          Rosetta™(DE3) Competent Cells - Novagen MSDS

          Title

          Safety Data Sheet (SDS) 

          Rosetta™(DE3) Competent Cells - Novagen Certificates of Analysis

          TitleLot Number
          70954

          Brochure

          Title
          Competent Cell Brochure

          Citations

          Title
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        • Priscilla Braglia, et al. (2007) Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae. Molecular and Cellular Biology 27, 1545-1557.
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        • Zubair M. Ahmed, et al. (2006) The tip-link antigen, a protein associated with the transduction complex of sensory hair cells, is protocadherin-15. Journal of Neuroscience 26, 7022-7034.
        • Kenji Kashiwagi, et al. (2006) Frame shuffling: a novel method for in vitro protein evolution. Protein Engineering Design and Selection 19, 135-140.
        • Amparo Lima, et al. (2006) A redox-active FKBP-type immunophilin functions in accumulation of the photosystem II supercomplex in Arabidopsis thaliana. Procedings of the National Academy of Science 103, 12631-12636.
        • Chad E. Metcalf and David A. Wassarman. (2006) DNA binding properties of TAF1 isoforms with two AT-hooks. Journal of Biological Chemistry 281, 30015-30023.
        • James Schelert, et al. (2006) Regulation of mercury resistance in the crenarchaeote Sulfolobus solfataricus. Journal of Bacteriology 188, 7141-7150.
        • Yi-Hsien Su and Victor D. Vacquier. (2006) Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa. Molecular Biology of the Cell 17, 114-121.
        • Guido Veit, et al. (2006) Collagen XXVIII, a novel von Willebrand Factor A domain-containing protein with many imperfections in the collagenous domain. Journal of Biological Chemistry 281, 3494-3504.
        • Jerome Vicogne, et al. (2006) Asymmetric phospholipid distribution drives in vitro reconstituted SNARE-dependent membrane fusion. Procedings of the National Academy of Science 103, 14761-14766.
        • Hao Xu and William Wickner. (2006) BEM1P is a positive regulator of the homotypic fusion of yeast vacuoles. Journal of Biological Chemistry 281, 27158-27166.
        • Philipp Beinker, et al. (2005) Biochemical coupling of the two nucleotide binding domains of ClpB: Covalent linkage is not a prerequisite for chaperone activity. Journal of Biological Chemistry 280, 37965-37973.
        • Eric R. Bonner, et al. (2005) Molecular basis of cysteine biosynthesis in plants: structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana. Journal of Biological Chemistry 280, 38803-38813.
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        • Johannes Haugstetter, Thomas Blicher and Lars Ellgaard. (2005) Identification and characterization of a novel thioredoxin-related transmembrane protein of the endoplasmic reticulum. Journal of Biological Chemistry 280, 8371-8380.
        • Meike Hoffmeister, et al. (2005) Mitochondrial trans-2-enoyl-coA reductase of wax ester fermentation from Euglena gracilis defines a new family of enzymes involved in lipid synthesis. Journal of Biological Chemistry 280, 4329-4338.
        • Shinpei Katou, et al. (2005) Catalytic activation of plant MAPK phosphatase NtMKP1 by its physiological substrate SIPK, but not by calmodulins. Journal of Biological Chemistry 280, 39569-39581.
        • Christopher K. Kennaway, et al. (2005) Dodecameric structure of the small heat shock protein Acr1 from Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 33419-33425.
        • Beth T. Vander Kooi, et al. (2005) The glucose-6-phosphatase catalytic subunit gene promoter contains both positive and negative glucocorticoid response elements. Molecular Endocrinology 19, 3001-3022.
        • Michael Kothe, et al. (2005) Direct demonstration of carbamoyl phosphate formation on the C-terminal domain of carbamoyl phosphate synthetase. Protein Science 14, 37-44.
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        • Peter A. Lemaire, Jeffrey Lary and James L. Cole. (2005) Mechanism of PKR activation: dimerization and kinase activation in the absence of double-stranded RNA. Journal of Molecular Biology 345, 81-90.
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        • User Protocols

          Title
          TB009 Competent Cells
          TB053 Academic and Non-profit Laboratory Assurance Letter

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