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555553 Rho Kinase Inhibitor III, Rockout - CAS 7272-84-6 - Calbiochem

The Rho Kinase Inhibitor III, Rockout, also referenced under CAS 7272-84-6, controls the biological activity of Rho Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-(4-Pyridyl)-1H-indole

Primary Target: ROCK
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
7272-84-6C₁₃H₁₀N₂

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      Description
      OverviewA cell-permeable indolopyridine compound that acts as a selective, reversible, and ATP-competitive inhibitor of Rho kinase activity with an IC50 of 25 µM. Does not inhibit the activation of Rho kinase nor does it affect the in vitro activities of MLCK, PKCα, and SAPK2a/p38α. Shown to be 5-fold less potent than Y-27632 (Cat. No. 688000; IC50 = ~5 µM), and display a similar specificity profile as H-89 (Cat. No. 371963). Affects cell migration, inhibits blebbing (IC50 = ~ 12 µM in M2 cells), and decreases stress fibers in Bulb 3T3 cells at 50 µM.
      Catalogue Number555553
      Brand Family Calbiochem®
      Synonyms3-(4-Pyridyl)-1H-indole
      References
      ReferencesYarrow, J.C., et al. 2005. Chem. Biol. 12, 385.
      Product Information
      CAS number7272-84-6
      ATP CompetitiveY
      FormYellow solid
      Hill FormulaC₁₃H₁₀N₂
      Chemical formulaC₁₃H₁₀N₂
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetROCK
      Primary Target IC<sub>50</sub>25 µM against ROCK
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      555553-10MG 04055977268430

      Documentation

      Rho Kinase Inhibitor III, Rockout - CAS 7272-84-6 - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      Rho Kinase Inhibitor III, Rockout - CAS 7272-84-6 - Calbiochem Certificates of Analysis

      TitleLot Number
      555553

      References

      Reference overview
      Yarrow, J.C., et al. 2005. Chem. Biol. 12, 385.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-January-2010 RFH
      Synonyms3-(4-Pyridyl)-1H-indole
      DescriptionA cell-permeable, selective, reversible, and ATP-competitive inhibitor of Rho kinase activity (IC50 = 25 µM). Does not inhibit the activation of Rho kinase nor does it affect the in vitro activities of MLCK, PKCα, and SAPK2a/p38α. Shown to be 5-fold less potent than Y-27632 (Cat. No. 688000; IC50 ~5 µM), and display a similar specificity profile as H-89 (Cat. No. 371963). Affects cell migration, inhibits blebbing (IC50 ~12 µM in M2 cells), and decreases stress fibers in Bulb 3T3 cells at 50 µM.
      FormYellow solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number7272-84-6
      Chemical formulaC₁₃H₁₀N₂
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (25 mg/ml) or methanol
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesYarrow, J.C., et al. 2005. Chem. Biol. 12, 385.