Millipore Sigma Vibrant Logo

QIA127 Rapid Cell Proliferation Kit

Rapid Cell Proliferation Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
QIA127
View Pricing & Availability

Overview

Key Spec Table

Detection Methods
Colorimetric

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
QIA127-1KIT
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Glass bottle 1 kit
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Add To Favorites
      Description
      OverviewAssay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
      Catalogue NumberQIA127
      Brand Family Calbiochem®
      Materials Required but Not Delivered• Pipettors with disposable tips
      • Tissue culture grade, flat-bottom 96-well plate
      • Spectrophotometer capable of measuring absorbance in 96-well plates at a wavelength of 440-460 nm
      References
      ReferencesIshiyama, M., et al. 1995. In Vitro Toxicology 8, 187.
      Liu, S.O., et al 1995. Nat. Med. 1, 267.
      Ishiyama, M., et al. 1993. Chem. Pharm. Bull. 41, 1118.
      Product Information
      Detection methodColorimetric
      Form500 Tests
      Format96-well plate
      Kit containsWST-1, 1-Methoxy PMS, and a user protocol.
      Quality LevelMQ100
      Biological Information
      Assay range1000 - 50,000 Cells
      Assay time0.5 - 4 h
      Sample TypeCultured cells
      Physicochemical Information
      Sensitivity1000 cells (adherent); 2500 cells (nonadherent)
      Product Usage Statements
      Intended useThe Calbiochem® Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Storage ConditionsUpon arrival store the entire contents of the kit at -20°C.
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsWST-1, 1-Methoxy PMS, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      QIA127-1KIT 07790788054021