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Protein Concentration and Desalting Prior to Screening Assays

Protein Concentration and Desalting Prior to Screening Assays

Overview

The MultiScreen filter plate with Ultracel-10 ultrafiltration membrane provides a high-throughput centrifugal system for sample purification, concen tration and desalting of biological solutions, as well as protein concentration and desalting prior to validation or screening assays.

The filter plate incorporates Ultracel-10 10,000 NMWL regenerated cellulose membrane for low protein binding and high protein retention. The system meets stringent manufacturing release specifications and is tested for >95% retention of Cytochrome c (12,500 daltons).

The MultiScreen filter plate with Ulracel-10 membrane allows for processing and collection of sample volumes from 50 µL to 500 µL. It is compatible with a range of standard 96-well microtiter receiver plates including 300 µL, 700 µL deep well and 150 µL conical well.

  • High throughput
  • Low binding
  • Tested for >95% retention of Cytochrome c

High Protein Retention

Membrane NMWL Protein Solute Typical Retention %
10,000 Cytochrome c
12,500 daltons (1 mg/mL)
>95
10,000 BSA
67,000 daltons (1 mg/mL)
>99
Performance of MultiScreen Filter Plate with Ultracel-10 membrane using a centrifuge at 2000 x g.