Millipore Sigma Vibrant Logo

382159 PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

Overview

Key Spec Table

Species ReactivityHostAntibody Type
Nem, Dr, H, T, XnRbPolyclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
382159-50UG
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Plastic ampoule 50 μg
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      Description
      OverviewRecognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
      Catalogue Number382159
      Brand Family Calbiochem®
      Application Data
      Detection of human histone H3, phosphorylated on Ser10, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer10) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
      References
      ReferencesAjiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
      Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
      Mahadevan, L.C., et al. 1991. Cell 65, 775.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Positive controlAcid-extracted proteins from colcemid-arrested HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunohistochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunohistochemistry (see comments)
      Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
      Application CommentsFor immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Nematode
      • Drosophila
      • Human
      • Tetrahymena
      • Xenopus
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      382159-50UG 04055977213218