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NB13 PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr²⁸⁶) (Ab-2) Mouse mAb (22B1)

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NB13
  
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      Overview

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~50 kDa α subunit of CaM kinase II phosphorylated at Thr286. Does not cross-react with unphosphorylated α subunit of CaM kinase II or with the β subunit in either phosphorylation state.
      Catalogue NumberNB13
      Brand Family Calbiochem®
      Application Data
      Detection of rat Ca2+/calmodulin kinase II α-subunit, phosphorylated on Thr286, by immunoblotting. Samples: Whole tissue extract from rat brain, unphosphorylated (lane 1) and phosphorylated (lane 2). Primary antibody: PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr286) (Ab-2) Mouse mAb (22B1) (Cat. No. NB13) (1:2000). Detection: chemiluminescence.
      References
      ReferencesMayford, M., et al. 1996. Science 274, 1678.
      Lisman, J. 1994. Trends Neurosci. 17, 406.
      Ochiishi, T., et al. 1994. Brain Res. 659, 179.
      Hanson, P.I. and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
      Silva, A.J., et al. 1992. Science 257, 201.
      Erondu, N.E. and Kennedy, M.B. 1985. J. Neurosci. 5, 3270.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesELISA (see comments)
      Immunoblotting (1:2000)
      Immunofluorescence (1:100)
      Application CommentsDoes not cross-react with the non-phosphorylated α subunit of CaM kinase II or the 60 kDa β subunit in either phosphorylation state. In rat hippocampal cells, this antibody labels cell somas and neuropil, while nuclei are only lightly stained by immunofluorescence. This antibody has been reported to work for ELISA. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide (MHRQEpTVDCLKKFN) corresponding to amino acids 281-294 surrounding the Thr²⁸⁶ phosphorylation site of mouse and rat CaM kinase II α subunit
      ImmunogenMouse and Rat
      Clone22B1
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      NB13 0