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480065 Necrostatin-1 - CAS 4311-88-0 - Calbiochem

Necrostatin-1, CAS 4311-88-0, is a cell-permeable, potent, and selective blocker of necroptosis (EC₅₀ = 494 nM in FADD-deficient Jurkat cells treated with TNF-α).

Necrostatin-1 - CAS 4311-88-0 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Necrosome Inhibitor I, 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, Nec-1, Necrosis Inhibitor II, Methyl-thiohydantoin-tryptophan, MTH-Trp, Receptor-Interacting Protein 1 Inhibitor I, RIPK 1 Inhibitor I, Nec1, RIP1 Inhibitor I

Primary Target: Blocker of necroptosis
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480065
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
4311-88-0C₁₃H₁₃N₃OS

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480065-5MG
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      Description
      OverviewA cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect of RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (Cat. No. 480066). Also available as a 25 mM solution in DMSO (Cat. No. 505224).
      Catalogue Number480065
      Brand Family Calbiochem®
      SynonymsNecrosome Inhibitor I, 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, Nec-1, Necrosis Inhibitor II, Methyl-thiohydantoin-tryptophan, MTH-Trp, Receptor-Interacting Protein 1 Inhibitor I, RIPK 1 Inhibitor I, Nec1, RIP1 Inhibitor I
      References
      ReferencesDegterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
      Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
      Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
      Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
      Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
      Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
      Muller, A.J., et al. 2005. Nat. Med. 11, 312.
      Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.
      Product Information
      CAS number4311-88-0
      ATP CompetitiveY
      FormYellow crystalline solid
      Hill FormulaC₁₃H₁₃N₃OS
      Chemical formulaC₁₃H₁₃N₃OS
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetBlocker of necroptosis
      Primary Target IC<sub>50</sub>EC50 = 494 nM in blocking necrosis in FADD-deficient Jurkat cells treated with TNF-α
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      480065-5MG 04055977201871

      Documentation

      Necrostatin-1 - CAS 4311-88-0 - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      Necrostatin-1 - CAS 4311-88-0 - Calbiochem Certificates of Analysis

      TitleLot Number
      480065

      References

      Reference overview
      Degterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
      Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
      Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
      Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
      Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
      Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
      Muller, A.J., et al. 2005. Nat. Med. 11, 312.
      Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-April-2008 JSW
      SynonymsNecrosome Inhibitor I, 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, Nec-1, Necrosis Inhibitor II, Methyl-thiohydantoin-tryptophan, MTH-Trp, Receptor-Interacting Protein 1 Inhibitor I, RIPK 1 Inhibitor I, Nec1, RIP1 Inhibitor I
      DescriptionA cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect of RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (Cat. No. 480066).
      FormYellow crystalline solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number4311-88-0
      Chemical formulaC₁₃H₁₃N₃OS
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (10 mg/ml) or Methanol (5 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesDegterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
      Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
      Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
      Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
      Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
      Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
      Muller, A.J., et al. 2005. Nat. Med. 11, 312.
      Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.