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MAB3328-25UG Ms X MMP-14 Antibody, clone LEM-2/15.8 [MT1-MMP]

Detect MMP-14 using this Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/15.8 validated for use in ELISA, IP & WB.

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MAB3328-25UG
25 μg  
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      Description
      Catalogue NumberMAB3328-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMs X MMP-14 Antibody, clone LEM-2/15.8 [MT1-MMP]
      Alternate Names
      • MT1-MMP
      Background InformationMT1-MMP plays an important role during endothelial cell migration and matrix remodeling. Although the role of MT1-MMP in endothelial cell motility is not fully characterized, its activity appears to modulate endothelial migration, invasion, and formation of capillary tubes during the angiogenic response (Galvez, 2001). Mt1-MMP also appears to play a key role in monocyte revruitment during inflammation (Salomon, 2005).
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin by Protein A chromatography . Liquid in 0.2M phosphate, 0.25M NaCl, pH 7.6, containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect MMP-14 using this Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/15.8 validated for use in ELISA, IP & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot

      Immunohistochemistry: Frozen and paraffin-embedded tissues

      Immunofluorescence

      Flow Cytometry

      Blocking: 10-15μg/mL

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide: amino acid sequence 218-233 within the catalytic domain
      Epitopecatalytic domain
      CloneLEM-2/15.8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityLEM-2/15.8 reacts with human MT1-MMP and displays crossreactivity with mouse specimens. This antibody was generated against the catalytic domain of MT1-MMP and is able to inhibit enzyme activity.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
      Gene Symbol
      • MMP14
      • MTMMP1
      • MMP-14
      • MT1MMP
      • MT1-MMP
      • MMP-X1
      • EC 3.4.24.80
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P50281 # Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
      COFACTOR: Binds 1 zinc ion per subunit (By similarity). & Calcium (By similarity).
      SIZE: 582 amino acids; 65884 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
      DOMAIN: SwissProt: P50281 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
      SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2° to 8°C for up to 12 months from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3328-25UG 04054839337444

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