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444232 MMP-9, Dimer, Human Neutrophil

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444232
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444232-5UG
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      Description
      OverviewDimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.
      M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.
      Catalogue Number444232
      Brand Family Calbiochem®
      SynonymsMatrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase
      References
      ReferencesOlson, M.W. et al. 2000. J. Biol. Chem. 275, 2661.
      Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.
      EC number3.4.24.35
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.
      Quality LevelMQ100
      Biological Information
      Purity≥90% by SDS-PAGE
      SourcePrepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Specific Activity≥1000 mU/mg protein
      Physicochemical Information
      ContaminantsNo other MMP activity detectable
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      444232-5UG 04055977186390