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427211 Lactate Dehydrogenase, Porcine Heart

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427211
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Overview

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427211-50KU
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      Description
      OverviewNative lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Note: 1KU = 1000 units.
      Note: 1 KU = 1000 units
      Catalogue Number427211
      Brand Family Calbiochem®
      References
      ReferencesKornberg, A. 1995. Methods Enzymol. 1, 441
      Product Information
      CAS number9001-60-9
      Unit of DefinitionOne unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
      EC number1.1.1.27
      FormLyophilized
      PI4.6
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥300 units/mg protein. One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
      Physicochemical Information
      ContaminantsGPT, GOT: ≤0.03%; PK, MK: ≤0.01%; MDH: ≤0.1%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      427211-50KU 04055977187397

      Documentation

      Lactate Dehydrogenase, Porcine Heart MSDS

      Title

      Safety Data Sheet (SDS) 

      Lactate Dehydrogenase, Porcine Heart Certificates of Analysis

      TitleLot Number
      427211

      References

      Reference overview
      Kornberg, A. 1995. Methods Enzymol. 1, 441
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-August-2009 JSW
      DescriptionNative lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.
      FormLyophilized
      CAS number9001-60-9
      EC number1.1.1.27
      ContaminantsGPT, GOT: ≤0.03%; PK, MK: ≤0.01%; MDH: ≤0.1%
      Specific activity≥300 units/mg protein. One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
      Unit definitionOne unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
      SolubilityAqueous Buffers
      Storage Protect from moisture
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.
      Toxicity Standard Handling
      Merck USA index14, 5334
      ReferencesKornberg, A. 1995. Methods Enzymol. 1, 441