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CBA010 InnoCyte™ Cell Migration Assay, 96-well

CBA010
  
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      Overview

      Key Spec Table

      Detection Methods
      Fluorometric
      Description
      Overview

      This product has been discontinued.



      A convenient assay for the chemotaxis cell migration. Cells migrate through a 8 μm pore to a feeder layer containing serum or other attractant. Migrated cells are quantitated using a cell-permeable fluorescent dye (Excitation max.: ~485 nm; Emission max.: ~520 nm).

      Catalogue NumberCBA010
      Brand Family Calbiochem®
      Application Data
      ~50,000 human umbilical venous endothelial cells (HUVECs) were incubated in triplicate wells of the upper chamber and allowed to migrate toward medium with or without chemotactic components in the lower chamber for 4 h in a 6% CO2 incubator at 37°C. The cells were detached and labeled as indicated in the Detailed Protocol. Latrunculin A, a powerful inhibitor of actin polymerization, was added to an aliquot of cell suspension immediately prior to loading the cells in the wells.

      ~60,000 HT-1080 cells were incubated in triplicate wells of the upper chamber and allowed to migrate toward medium with or without serum for 3 h in a 6% CO2 incubator at 37°C. The cells were detached and labeled as indicated in the Detailed Protocol.
      Materials Required but Not Delivered Pipettors with disposable tips
      Black 96-well strips or black plate
      Phosphate buffered saline (PBS)
      Fluorescence reader capable of measuring fluorescence in 96-well plates at an excitation wavelength of ~485 nm and an emission wavelength of ~520 nm
      References
      ReferencesKlinghoffer, R.A., et al. 1999. EMBO J. 18, 2459.
      Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677.
      Smilenov, L. B., et al. 1999. Science 286, 1172.
      Burridge, K., et al. 1997. Trends Cell Biol. 7, 342.
      Huttenlocher, A., et al. 1997. J. Biol. Chem. 272, 32719.
      Lauffenburger, D.A., and Horowitz, A.F. 1996. Cell 84, 359.
      Product Information
      Detection methodFluorometric
      Form96 Tests
      Format96-well plate
      Kit containsSterile 96-Well Migration Chamber, Culture Tray, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol.
      Quality LevelMQ100
      Applications
      Application ReferencesLoberg, R.D., et al. 2006. Neoplasia 8, 578.
      Biological Information
      Assay time3-5 h
      Sample TypeAdherent cells
      Product Usage Statements
      Intended useThe Calbiochem® InnoCyte™ Cell Migration Assay is designed to study the effects of various drugs or agents on cell motility or for identifying chemoattractant molecules (chemotactic migration). The assay is not intended for leukocyte migration experiments, as the study of leukocytes requires membranes with a pore-size smaller than 8 µm.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Storage ConditionsUpon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsSterile 96-Well Migration Chamber, Culture Tray, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      CBA010 0