Millipore Sigma Vibrant Logo

IEVH85R Immobilon®-E PVDF Membrane

The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

View Products on Sigmaaldrich.com
IEVH85R
1  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Catalogue NumberIEVH85R
      Trade Name
      • Immobilon
      DescriptionImmobilon®-E PVDF Membrane
      OverviewImmobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.
      Background InformationThis 8.5cm x 10m roll offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm). It works with the optional Immobilon NOW Dispenser (catalog number IMDISP).

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
      - Compatible with chemiluminescent and chromogenic detection

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.

      Features & Benefits:
      - High protein binding provides stronger signal
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be stripped and reprobed multiple times
      References
      Product Information
      Detection methodChemiluminescence Chromogenic Radioactive
      HS Code3921 19 00
      Compatible Stains
      • Coomassie brilliant blue
      • Amido black
      • India ink
      • Ponceau-S red
      • Colloidal gold
      • Transillumination
      • Sypro® ruby
      Filter CodeIEVH
      Filter ColorWhite
      Quality LevelMQ400
      Applications
      ApplicationThe first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
      Key Applications
      • Western Blotting
      • Immunoblotting (Western)
      Biological Information
      Primary Binding MechanismsElectrostatic, hydrophobic
      BSA AdsorptionNot Applicable
      WettabilityEasily wetted with water
      Physicochemical Information
      Pore Size0.45 µm
      Dimensions
      Filter SurfacePlain
      Filter Dimensions8.5 cm x 10 m
      Materials Information
      Chemistry
      • Polyvinylidene Fluoride (PVDF)
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size1
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IEVH85R 04054839569098

      Related Products & Applications

      Product Families

      Immobilon® Membranes, Sandwiches and Blotting Filter Paper

      Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.
      Learn More >>