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345762
Sigma-AldrichGDF-8/Myostatin, Human, Mouse, Rat, Recombinant
GDF-8/Myostatin, Human, Mouse, Rat, Recombinant, consists of amino acids 268-376 and is expressed in NS0-derived murine myeloma cells. Useful for inhibiting preadipocyte differentiation to adipocytes.
More>>GDF-8/Myostatin, Human, Mouse, Rat, Recombinant, consists of amino acids 268-376 and is expressed in NS0-derived murine myeloma cells. Useful for inhibiting preadipocyte differentiation to adipocytes. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recombinant, human mouse rat growth differentiation factor 8 (GDF-8) consisting of amino acids 268-376 and expressed in NS0-derived murine myeloma cells. GDF-8 is expressed in developing and adult skeletal muscle and is 100% identical to mature human, rat, mouse, and cow at the amino acid sequence level. Useful for the inhibition of preadipocyte differentiation to adipocytes.
Catalogue Number
345762
Brand Family
Calbiochem®
Synonyms
Myostatin
References
References
Kim, H.S., et al. 2001. Biochem. Biophys. Res. Commun.281, 902. Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA98, 9306. Sharma, M., et al. 1999. J. Cell Physiol.180, 1. McPherron, A.C., et al. 1997. Nature387, 83. Storm, E.E., et al. 1994. Nature368, 639.
Product Information
Form
Solid
Formulation
Lyophilized from 0.2 µm filtered Acetonitrile, TFA, BSA.
GDF-8/Myostatin, Human, Mouse, Rat, Recombinant, consists of amino acids 268-376 and is expressed in NS0-derived murine myeloma cells. Useful for inhibiting preadipocyte differentiation to adipocytes.
Biological Information
Biological activity
Hemoglobin Expression: ED₅₀ = 2-10 ng/ml as measured by induction of hemoglobin expression in k562 human chronic myelogenous leukemia cells.
Elongation of Animal Caps: Optimal Elongation of animal caps from Xenpus blastulae.
Purity
≥80% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: <1.0 EU per/µg protein
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Global Trade Item Number
Catalogue Number
GTIN
345762-10UG
04055977193954
Documentation
GDF-8/Myostatin, Human, Mouse, Rat, Recombinant MSDS
GDF-8/Myostatin, Human, Mouse, Rat, Recombinant Certificates of Analysis
Title
Lot Number
345762
References
Reference overview
Kim, H.S., et al. 2001. Biochem. Biophys. Res. Commun.281, 902. Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA98, 9306. Sharma, M., et al. 1999. J. Cell Physiol.180, 1. McPherron, A.C., et al. 1997. Nature387, 83. Storm, E.E., et al. 1994. Nature368, 639.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
12-September-2011 RFH
Synonyms
Myostatin
Description
Recombinant, human mouse rat growth differentiation factor 8 (GDF-8) consisting of amino acids 268-376 and expressed in NS0-derived murine myeloma cells. GDF-8 is expressed in developing and adult skeletal muscle and is 100% identical to mature human, rat, mouse, and cow at the amino acid sequence level. Useful for the inhibition of preadipocyte differentiation to adipocytes.
Form
Solid
Formulation
Lyophilized from 0.2 µm filtered Acetonitrile, TFA, BSA.
Purity
≥80% by SDS-PAGE
Contaminants
Endotoxin: <1.0 EU per/µg protein
Biological activity
Hemoglobin Expression: ED₅₀ = 2-10 ng/ml as measured by induction of hemoglobin expression in k562 human chronic myelogenous leukemia cells.
Elongation of Animal Caps: Optimal Elongation of animal caps from Xenpus blastulae.
Solubility
Reconstitute to 100 µg/ml in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Kim, H.S., et al. 2001. Biochem. Biophys. Res. Commun.281, 902. Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA98, 9306. Sharma, M., et al. 1999. J. Cell Physiol.180, 1. McPherron, A.C., et al. 1997. Nature387, 83. Storm, E.E., et al. 1994. Nature368, 639.