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14-725M EGFR (T790M) Protein, active, 250 µg

Active, N-terminal GST-tagged recombinant human EGF Receptor, amino acids 696end containing the mutation T790M, for use in Enzyme Assays.

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14-725M
250 µg  Also available in 10 μg size (cat#14-725) and in bulk (cat#14-725-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-725M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionEGFR (T790M) Protein, active, 250 µg
      OverviewN-terminal GST-tagged recombinant human EGF Receptor, amino acids 696end containing the mutation T790M
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, N-terminal GST-tagged recombinant human EGF Receptor, amino acids 696end containing the mutation T790M, for use in Enzyme Assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Sourceexpressed by baculovirus in Sf21 cells
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Protein TargetEGFR
      Purification MethodGlutathione-agarose
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight86kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of 250μM substrate peptide (GGMEDIYFEFMGGKKK)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C from date of shipment
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-725) and in bulk (cat#14-725-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-725M 04053252925450

      Documentation

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-725 EGFR (T790M) Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases