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ECM545 Sigma-AldrichECM Cell Adhesion Array Kit, fluorometric

The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

ECM Cell Adhesion Array Kit, fluorometric MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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Technical Information

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ECM545

1 plate 96 wells

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Key Spec Table

Detection Methods
Fluorescent

Description
Catalogue Number	ECM545
Brand Family	Chemicon®
Trade Name	Chemicon
Description	ECM Cell Adhesion Array Kit, fluorometric
Overview	Introduction: Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON® ECM Cell Adhesion Array Kit (Fluorometric) provides an ECM Array microtiter plate with a homogenous fluorescence detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion. In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including: · Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530) · Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531) · Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532) · Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM533) · Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM534) · Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorometric) (ECM535) · ECM Cell Adhesion Array Kit (Colorimetric) (ECM540) · QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510) · QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512) · QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515) · QCM™ 96-well Cell Invasion Assay (ECM555) · QCM™ 96-well Collagen-Based Cell Invasion Assay (ECM 556) · 24-well Insert Cell Migration and Invasion assay systems · CytoMatrix™ Cell Adhesion strips (ECM protein coated) · QuantiMatrix™ ECM protein ELISA kits Test Principle: The CHEMICON® ECM Cell Adhesion Array kit utilizes a homogenous fluorescence detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are lysed and detected by the patented CyQuant GR® dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3). Relative cell attachment is determined using a fluorescence plate reader. Additional information about the ECM components provided in the kit:
Materials Required but Not Delivered	· Multi-channel and/or repeating pipettes. · Harvesting buffer: EDTA or trypsin cell detachment buffer. Suggested formulations include a) 2 mM EDTA/PBS, b) 0.05% trypsin in Hanks Balanced Salt Solution (HBSS) containing 25 mM HEPES, or other cell detachment formulations as optimized by individual investigators. Note: Trypsin cell detachment buffer maybe required for difficult cell lines. Allow sufficient time for cell receptor recovery. · Tissue culture growth medium appropriate for subject cells, such as DMEM containing 10% FBS. · Quenching Medium: serum-free medium, such as DMEM, EMEM, or FBM (fibroblast basal media), containing 5% BSA. Note: Quenching Medium must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in the harvesting buffer. · Sterile PBS or HBSS to wash cells. · Deionized water. · Low speed centrifuge and tubes for cell harvesting. · CO2 incubator appropriate for subject cells. · Sterile cell culture hood. · Hemacytometer and microscope for counting cells. · Trypan Blue or equivalent viability stain. · Fluorescence plate reader with 485nm and 530nm filter set. · 96-well plate suitable for fluorescence measurement.

References

Product Information
Components	ECM Array Plate: (PN: 90652) One 96-well plate with 12 strips. Each 8-well strip consists of 7 different human, ECM protein-coated wells (Collagen I, Collagen II, Collagen IV, Fibronectin, Laminin, Tenascin, Vitronectin) and one BSA-coated well (negative control). See the plate layout on data sheet. 4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL. CyQuant GR® Dye: (Part No. 90132) One vial - 75 μL. Assay Buffer: (Part No. 90601) One bottle - 100 mL.
Detection method	Fluorescent
Quality Level	MQ100

Applications
Application	The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.
Application Notes	The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors. For Research Use Only. Not for use in diagnostic procedures.

Biological Information
Species Reactivity	Human

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	The ECM Array Plate can be stored at 2° to 8°C in the foil pouch up to its expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C. Precautions: No data is available on the biological toxicity of CyQuant GRâ dye. This reagent binds to nucleic acids, and as such, should be treated as a potential mutagen which may cause cancer and heritable genetic damage. Handle with caution. The DMSO stock solution should be handled with special caution as DMSO can facilitate the entry of organic molecules into tissues.

Storage and Shipping Information

Storage Conditions

The ECM Array Plate can be stored at 2° to 8°C in the foil pouch up to its expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.

Precautions:

No data is available on the biological toxicity of CyQuant GRâ dye. This reagent binds to nucleic acids, and as such, should be treated as a potential mutagen which may cause cancer and heritable genetic damage. Handle with caution. The DMSO stock solution should be handled with special caution as DMSO can facilitate the entry of organic molecules into tissues.

Packaging Information
Material Size	1 plate
Material Package	96 wells

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
ECM545	04053252675393

Documentation

ECM Cell Adhesion Array Kit, fluorometric MSDS

Title
Safety Data Sheet (SDS)

References

Reference overview	Pub Med ID
Transmembrane motility assay of transiently transfected cells by fluorescent cell counting and luciferase measurement. Gildea, J J, et al. BioTechniques, 29: 81-6 (2000) 2000 Show Abstract Current in vitro assays used in assessing tumor motility could be improved by the development of a simple technique that would facilitate studies of the impact of specific genes on pharmacologically altered chemotaxis. We developed a technique that improves on the classic transwell assay by using fluorescence and luminescence to assess chemotaxis. In this transient transfection system, co-transfection of a reporter construct and a gene with an unknown impact on motility are coupled with biochemical assays to quantitate the number of cells that have received a transferred gene, which subsequently crosses the membrane. This assay was found to be less variable than the conventional transwell chamber and is easily adaptable to studies of cell motility or cell invasion. We also demonstrate that this assay can detect the effect of both genetic and pharmacological inhibition of motility alone and in combination. It therefore has the potential to reveal additive or synergistic effects.	10907081

Reference overview

Pub Med ID

Technical Info

Title
Integrin Profiling of Stem Cells and Differentiated Progeny
Screening Kits to Monitor Cell - Extracellular Matrix Interactions

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ECM545 Sigma-AldrichECM Cell Adhesion Array Kit, fluorometric

The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

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