69725 Sigma-AldrichλDE3 Lysogenization Kit plus pLysS & pLysE - Novagen
Recommended Products
Overview
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Pricing & Availability
| Catalogue Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
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| 69725-3 |
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Glass bottle | 10 rxn |
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| Description | |
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| Overview | The λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis. |
| This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
| Catalogue Number | 69725 |
| Brand Family | Novagen® |
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| Product Information | ||
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| • | 200 µl | λDE3 Phage Lysate |
| • | 200 µl | Helper Phage Lysate |
| • | 200 µl | Selection Phage Lysate |
| • | 50 µl | T7 Tester Phage Lysate |
| • | 200 µl | HMS174(DE3) glycerol stock |
| • | 1 µg | pLysS DNA |
| • | 1 µg | pLysE DNA |
| Quality Level | MQ100 | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 69725-3 | 04055977258981 |
Documentation
λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen MSDS
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λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen Certificates of Analysis
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| 69725 |
Citations
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User Protocols
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| TB053 Academic and Non-profit Laboratory Assurance Letter |
Vector Map
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| TB107 pLysS and pLysE Plasmid Map |