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251765 DAR-4M AM - Calbiochem

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

DAR-4M AM - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Diaminorhodamine-4M AM

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251765
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₈H₃₁IN₄O₅

Pricing & Availability

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251765-1MG
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      		 Plastic ampoule 1 mg
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      Description
      OverviewA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.
      Catalogue Number251765
      Brand Family Calbiochem®
      SynonymsDiaminorhodamine-4M AM
      References
      ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
      Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
      Product Information
      ATP CompetitiveN
      DeclarationNot available for sale in Japan.
      FormDark red solution
      FormulationIn DMSO.
      Hill FormulaC₂₈H₃₁IN₄O₅
      Chemical formulaC₂₈H₃₁IN₄O₅
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetNitric oxide (NO) fluorescent indicator
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 23-26-36

      Do not breathe fumes.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      251765-1MG 04055977217261

      Documentation

      DAR-4M AM - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      DAR-4M AM - Calbiochem Certificates of Analysis

      TitleLot Number
      251765

      References

      Reference overview
      Kojima, H., et al. 2001. Anal. Chem. 73, 1967.
      Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-November-2007 JSW
      SynonymsDiaminorhodamine-4M AM
      DescriptionA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.
      FormDark red solution
      FormulationIn DMSO.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions
      This product is provided at a concentration of ~5 mM. Prepare a 500-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol red may affect fluorescence.
      Chemical formulaC₂₈H₃₁IN₄O₅
      Structure formulaStructure formula
      Purity≥98% by HPLC
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.
      Toxicity Irritant
      ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
      Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.