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70526 Clonables™ Ligation/Transformation Kit - Novagen

Simple, reproducible ligation and transformation—in as little as 23 minutes

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70526
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Overview

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70526-3
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      		 Plastic ampoule 11 rxn
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      Description
      OverviewThe Clonables™ Kit enables convenient, dependable, high-efficiency ligation and transformation of any compatible DNA ends. The kit features a unique, universal Ligation Premix, containing ligase, buffer, and cofactors, which supports ligation of any type of DNA cohesive or blunt ends in a 15-minute reaction. Ligated DNA is transformed into NovaBlue Singles™ Competent Cells, which use a streamlined protocol that takes less than 8 minutes for ampicillin-resistant plasmids and 38 minutes for other antibiotic-resistant plasmids. This kit can be used with a variety of cloning vectors and is compatible with any type of DNA end, without altering the ends or desired cloning junctions.

      The Clonables Kit contains sufficient reagents to perform 11 ligation and transformation reactions, and includes a control vector and insert mix to verify performance.
      Catalogue Number70526
      Brand Family Novagen®
      Features and benefits
    • Fast! 15-minute ligation, 8-minute transformation with ampicillin selection
    • Ligation components premixed to decrease pipetting steps and increase reliability
    • One reaction, optimized for cohesive ends, single base overhangs, and blunt ends
    • Single-use competent cells eliminate need to aliquot, freeze/thaw, or waste partially used vials
    • Compatible with PCR buffer, TE, restriction enzyme buffer, or End Conversion Mix
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      References
      Product Information
      Components
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70526-3 04055977273595

      Documentation

      Clonables™ Ligation/Transformation Kit - Novagen Certificates of Analysis

      TitleLot Number
      70526

      Brochure

      Title
      High fidelity gene amplification

      Citations

      Title
    • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
    • Bratislav Janjic, et al. (2006) Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. Journal of Immunology 177, 2717-2727.
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      User Protocols

      Title
      TB233 Clonables™ Kit