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218785 Caspase-10, Human, Recombinant, E. coli

Caspase-10, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag® sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mch4, FLICE-2

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218785
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Overview

Replacement Information

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218785-3000U
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      		 Plastic ampoule 3000 u
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      Description
      OverviewRecombinant, human caspase-10 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
      M.W. 11,000 and 18,000 (heterodimer).
      Catalogue Number218785
      Brand Family Calbiochem®
      SynonymsMch4, FLICE-2
      References
      ReferencesKang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
      Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
      Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
      Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
      Product Information
      Activity≥50,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=368057">Cat. No. 368057</a>) per min at 25°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥150,000 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      218785-3000U 04055977218763

      Documentation

      Caspase-10, Human, Recombinant, E. coli MSDS

      Title

      Safety Data Sheet (SDS) 

      Caspase-10, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      218785

      References

      Reference overview
      Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
      Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
      Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
      Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-September-2007 RFH
      SynonymsMch4, FLICE-2
      DescriptionRecombinant, human caspase-10 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.
      Purity≥95% by SDS-PAGE
      Specific activity≥150,000 units/mg protein
      Activity≥50,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesKang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
      Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
      Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
      Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.