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208718 Calpain-2, Rat, Recombinant, High Purity, E. coli

208718
  
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      Overview

      Description
      Overview

      This product has been discontinued.



      Recombinant, rat calpain-2 expressed in E. coli. Ca2+-dependent heterodimeric cysteine proteinase with high Ca2+ requirement (EC50 = 350 µM Ca2+). Supplied as highly purified preparation.
      Catalogue Number208718
      Brand Family Calbiochem®
      Synonymsm-Calpain
      References
      ReferencesElce, J.S., et al. 1995. Protein Eng. 8, 843.
      Graham-Siegenthaler, K., et al. 1994. J. Biol. Chem. 269, 30457.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 pmol. Suc-leu-Leu0Val-Tyr-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorometric (Cat. No. QIA120). Note: 1 caseinolytic unit = 0.8 fluorogenic units.
      EC number3.4.22.17
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 5 mM β-mercaptoethanol, 40% glycerol, pH 7.8.
      Biological Information
      Purity≥98% by SDS-PAGE
      Specific Activity≥1500 units/mg protein
      Safety Information
      R PhraseR: 36/37/38-42/43

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Harmful
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      208718 0