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OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP33
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, Pm, RMMonoclonal Antibody

Pricing & Availability

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OP33-100UG
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      OP33-20UG
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      Limited Availability Limited Availability
      In Stock 
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      Limited Quantities Available
      Availability to be confirmed
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          Description
          OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
          Catalogue NumberOP33
          Brand Family Calbiochem®
          References
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Ball, R.K., et al. 1984. EMBO J. 3, 1485.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Positive controlHs27 cells or breast carcinoma tissue
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
          Key Applications Frozen Sections
          Immunofluorescence
          Immunoprecipitation
          Not Western Blot
          Paraffin Sections
          Application NotesFrozen Sections (see applications references)
          Immunoblotting (not recommended)
          Immunofluorescence (1-20 μg/ml)
          Immunoprecipitation (1 μg per sample)
          Paraffin Sections (5 μg/ml, heat pre-treatment required)
          Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          ImmunogenvLM mouse tumor cells
          ImmunogenMouse
          ClonePAb1620
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Bovine
          • Human
          • Mouse
          • Primate
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          OP33-100UG 04055977208443
          OP33-20UG 07790788053901

          Documentation

          Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) Certificates of Analysis

          TitleLot Number
          OP33

          References

          Reference overview
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Ball, R.K., et al. 1984. EMBO J. 3, 1485.

          Brochure

          Title
          Caspases and other Apoptosis Related Tools Brochure

          Citations

          Title
        • Pavel Kramata, et al. (2005) Patches of Mutant p53-Immunoreactive Epidermal Cells Induced by Chronic UVB Irradiation Harbor the Same p53 Mutations as Squamous Cell Carcinomas in the Skin in Hairless SKH-1 Mice. Cancer Research 65, 3577-3585.
          		•
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2007 RFH
          ApplicationFrozen Sections (see applications references)
          Immunoblotting (not recommended)
          Immunofluorescence (1-20 μg/ml)
          Immunoprecipitation (1 μg per sample)
          Paraffin Sections (5 μg/ml, heat pre-treatment required)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~53 kDa wild-type p53 protein.
          BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phospho-protein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
          HostMouse
          Immunogen speciesMouse
          ImmunogenvLM mouse tumor cells
          ClonePAb1620
          IsotypeIgG2a
          Speciesbovine, human, mouse, primate, rat
          Positive controlHs27 cells or breast carcinoma tissue
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Ball, R.K., et al. 1984. EMBO J. 3, 1485.
          Citation
        • Pavel Kramata, et al. (2005) Patches of Mutant p53-Immunoreactive Epidermal Cells Induced by Chronic UVB Irradiation Harbor the Same p53 Mutations as Squamous Cell Carcinomas in the Skin in Hairless SKH-1 Mice. Cancer Research 65, 3577-3585.
          		•
          Application referencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies