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MABE1828-25UL Anti-beta-Catenin Antibody, clone mAb-Cat1

Anti-beta-Catenin, clone mAb-Cat1, Cat. No. MABE1828, is a highly specific rat monoclonal antibody that targets beta-catenin 1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Dot

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Anti-beta-Catenin Antibody, clone mAb-Cat1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CTNNB1, CTNNB, OK/sw-cl.35, PRO2286 UniProt Number: P35222
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MABE1828-25UL
25 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HChIP, DB, IP, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1828-25UL
      DescriptionAnti-beta-Catenin Antibody, clone mAb-Cat1
      Alternate Names
      • beta-Catenin 1
      • Catenin beta-1
      Background InformationCatenin beta-1 (UniProt: P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human. Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC beta-catenin is trimethylated on Lysine 49 by Ezh2 methyltransferase and this trimethylated form represses neuronal differentiation genes Sox1 and Sox3. Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824.
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-beta-Catenin, clone mAb-Cat1, Cat. No. MABE1828, is a highly specific rat monoclonal antibody that targets beta-catenin 1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunoprecipitation, and Western Blottiing.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Dot Blot
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

      Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

      Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
      Biological Information
      ImmunogenBSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
      ClonemAb-Cat1
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityThis rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine and Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTNNB1
      • CTNNB
      • OK/sw-cl.35
      • PRO2286
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~100 kDa observed; 85.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SW 480 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected beta-Catenin in SW 480 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1828-25UL 04054839589683