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MABF2676-25UL Anti-TCR Vγ7 Antibody, clone F2.67

Anti-TCR Vg7, clone F2.67, Cat. No. MABF2676, is a mouse monoclonal antibody that detects T cell receptor g, variable 7 and is tested for use in Flow Cytometry.

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MABF2676-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2676-25UL
      DescriptionAnti-TCR Vγ7 Antibody, clone F2.67
      Alternate Names
      • T cell receptor gamma
      • variable 7
      Background InformationT cell receptor gamma, variable 7 (UniProt: A0A0A6YYE8; also known as TCR Vg7) is encoded by the Trgv7 (also known as Tcrg-V7) gene (Gene ID: 21641) in murine species. The T-cell receptor (TCR) is a protein complex found on the surface of T cells that is responsible for recognizing fragments of antigen as peptides bound to major histocompatibility complex (MHC) molecules. The TCR is a heterodimer of a and b chain and over 95% of T cells express these two chains and only about 5% of T cells carry g and d chains, which are the first T lymphocytes of innate immunity to arise in ontogeny. The diversity of TCR arises from genetic recombination of the DNA-encoded segments in individual somatic T cells by somatic V(D)J recombination using RAG1 and RAG2 recombinases. This recombination process is reported to be unique to lymphocytes during the early stages of their development in primary lymphoid organs. The generation of the TCR g chain involves VJ recombination. There are seven Vg gene segments have been described in the TCRg chain loci of mice. TCR Vg7 is localized on the external side of plasma membrane. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce the mature form. In most inbred strains of mice, T cells expressing Vg7- or Vg1- encoded TCRs make up 90% of intestinal intraepithelial lymphocytes. These cells are reported to be essential to limit parasite invasion and their dissemination from the intestinal mucosa. (Ref.: Li, C., et al. (2012). Cell. Immunol. 279(2); 151-159; Dalton, JE., et al. (2006). Gatroenterol.131(3); 818-829; Pereira, ,P., et al. (2000). Eur. J. Immunol. 30(7); 1988-1997).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-TCR Vg7, clone F2.67, Cat. No. MABF2676, is a mouse monoclonal antibody that detects T cell receptor g, variable 7 and is tested for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected TCR Vg7 in Flow Cytometry applications (Pereira, P., et al. (2000). Eur J Immunol.;30(7):1988-97; Dalton, J.E., et al. (2006). Gastroenterology.;131(3):818-29; Newton, D.J., et al. (2006). Infect Immun.;74(2):1097-105; Hamada, S. et al. (2008). Immunology.;125(2):170-7; Li, C., et al. (2012). Cell Immunol.;279(2):151-9; Walker, C.R., et al. (2013). PLoS One.;8(12):e84553).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenMouse hybridoma expressing anti V 7V 5 TCR.
      EpitopeUnknown
      CloneF2.67
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone F2.67 is a mouse monoclonal antibody generated in TCR delta knockout mice. It detects murine T cell receptor g, variable 7.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Trgv7
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight13.53 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in Balb/c mouse thymocytes.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected T-cell Vg 7 in one million Balb/c mouse thymocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2676-25UL 04065266060096