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MABE1923-25UG Anti-T2A Antibody, clone 5E6

Anti-T2A, clone 5E6, Cat. No. MABE1923, is a rat monoclonal antibody that detects T2A and is tested for use in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.

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MABE1923-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1923-25UG
      DescriptionAnti-T2A Antibody, clone 5E6
      Alternate Names
      • Thosea asigna virus 2A
      Background InformationThosea asigna virus (TaV), a member of the genus Betatetravirus of the family Tetraviridae, is a positive-sense single-stranded RNA virus that infects lepidopteran insects. This family is divided into the Betatetravirus and Omegatetravirus genera, based upon of the appearance of the virion and genome organization. 2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 16-20 amino acids (depending on the virus of origin). They contain a highly conserved sequence, GDVEXNPGP, at the C-terminus, which is shared by different 2As and is essential for the creation of steric hindrance and ribosome skipping. These peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multi-cistronic construct. These peptides are used by several families of viruses, the best-known amongst these are foot-and-mouth disease virus 2A (F2A) and Thosea asigna virus 2A (T2A). Clone 5E6 is a rat monoclonal antibody that reacts with a protein in lysate from HEK293T cells transfected with various 2A-tagged proteins.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-T2A, clone 5E6, Cat. No. MABE1923, is a rat monoclonal antibody that detects T2A and is tested for use in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry
      • ELISA
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected T2A tagged proteins in Western Blotting applications (Lange, A., et al. (2012). Differentiation.;83(2):S105-13).

      Immunofluorescence Analysis: A 1:200 dilution of this antibody detected T2A tagged proteins in mouse E14.5 lung and pancreas tissue sections (Courtesy of Dr. Ingo Burtscher, Ph.D., Helmholtz Zentrum München, Germany).

      Immunohistochemistry Applications: A representative lot detected T2A tagged proteins in Immunohistochemistry applications (Lange, A., et al. (2012). Differentiation.;83(2):S105-13).

      Immunofluorescence Analysis: A representative lot detected T2A tagged proteins in Immunofluorescence applications (Lange, A., et al. (2012). Differentiation.;83(2):S105-13).

      ELISA Analysis: A representative lot detected T2A tagged protein in ELISA applications (Lange, A., et al. (2012). Differentiation.;83(2):S105-13).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to 17 amino acids from Thosea asigna T2A protein.
      EpitopeC-terminal
      Clone5E6
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 5E6 is a rat monoclonal antibody that detects T2A tagged protein.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteAll.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells transfected with alpha-Tubulin vector.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected T2A tagged alpha-Tubulin in lysate from HEK293 cells transfected with alpha-Tubulin vector.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1923-25UG 04065266908220

      Documentation

      Anti-T2A Antibody, clone 5E6 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-T2A Antibody, clone 5E6 Certificates of Analysis

      TitleLot Number
      Anti-T2A, clone 5E6 - 3995304 3995304
      Anti-T2A, clone 5E6 - 4014617 4014617
      Anti-T2A, clone 5E6 - 4017106 4017106
      Anti-T2A, clone 5E6 - 4032058 4032058
      Anti-T2A, clone 5E6 - 4054124 4054124
      Anti-T2A, clone 5E6 - 4074329 4074329
      Anti-T2A, clone 5E6 - 4093629 4093629
      Anti-T2A, clone 5E6 - 4096085 4096085
      Anti-T2A, clone 5E6 - 4107274 4107274
      Anti-T2A, clone 5E6 - 4107744 4107744
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