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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).
More>>This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622). Less<<
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Description
Overview
Recognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
Catalogue Number
PC177L
Brand Family
Calbiochem®
Application Data
Detection of rat serotonin (5-HT) transporter by immunohistochemistry. Sample: Rat raphe nuclei, hypthalamus, cortex, and spinal cord. Primary antibody: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb (Cat. No. PC177L) (1:1000). Detection: fluorescence.
References
References
Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267. Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263. Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
Product Information
Form
Lyophilized
Formulation
Undiluted serum.
Positive control
Rat naphe nuclei, hypothalamus, cortex, or spinal cord
Frozen Sections (1:10,000-1:15,000; see application references) Immunoblotting (see comments) Immunofluorescence (1:200-1:400)
Application Comments
Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
Immunogen
Rat
Host
Rabbit
Isotype
IgG
Species Reactivity
Mouse
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb Certificates of Analysis
Title
Lot Number
PC177L
References
Reference overview
Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267. Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263. Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
05-June-2008 JSW
Application
Frozen Sections (1:10,000-1:15,000; see application references) Immunoblotting (see comments) Immunofluorescence (1:200-1:400)
Application Data
Detection of rat serotonin (5-HT) transporter by immunohistochemistry. Sample: Rat raphe nuclei, hypthalamus, cortex, and spinal cord. Primary antibody: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb (Cat. No. PC177L) (1:1000). Detection: fluorescence.
Description
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.
Background
The serotonin (5-hydroxytryptamine; 5-HT) transporter (5-HTT) plays an important role in the re-uptake of serotonin from the nerve terminal to ultimately terminate the serotonin signal. The 5-HTT plays a critical role in the regulation of serotonin neurotransmission, which has been linked to mood and personality disorders, anxiety, eating disorders, and insomnia. The predicted 12 transmembrane serotonin transporter contains potential sites for phosphorylation and glycosylation. Because serotonin is involved in such a variety of biological processes, the 5-HTT has been a target for the development of antidepressants and psychostimulants. Blocking serotonin uptake at the synapse has been achieved using structurally related chemicals called Selective Serotonin Re-uptake Inhibitors (SSRI) which alleviate disorders such as depression and alcoholism. The 5-HTT is localized to the plasma membrane of neurons and glial cells, as well as blood platelets, placenta, and endothelial cells.
Host
Rabbit
Immunogen species
Rat
Immunogen
a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
Isotype
IgG
Species
mouse, rat
Positive control
Rat naphe nuclei, hypothalamus, cortex, or spinal cord
Form
Lyophilized
Formulation
Undiluted serum.
Preservative
≤0.1% sodium azide
Comments
Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
Toxicity
Standard Handling
References
Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267. Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263. Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
Application references
Frozen Sections, Mouse Cross-Reactivity
Salichon N., et al. 2001. J. Neuerosci.21, 884.
