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05-1078 Anti-STAT3 Antibody, clone 18F7.1

Use Anti-STAT3 Antibody, clone 18F7.1 (mouse monoclonal antibody) validated in WB, ICC to detect STAT3 also known as Acute-phase response factor, DNA-binding protein APRF, signal transducer & activator of transcription 3.

Anti-STAT3 Antibody, clone 18F7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: APRF, FLJ20882, HIES, MGC16063 UniProt Number: P40763
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05-1078
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1078
      Replaces04-1014
      DescriptionAnti-STAT3 Antibody, clone 18F7.1
      Alternate Names
      • Acute-phase response factor
        DNA-binding protein APRF
        signal transducer and activator of transcription 3
        signal transducer and activator of transcription 3
        (acute-phase response factor)
      Background InformationSTAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-STAT3 Antibody, clone 18F7.1 (mouse monoclonal antibody) validated in WB, ICC to detect STAT3 also known as Acute-phase response factor, DNA-binding protein APRF, signal transducer & activator of transcription 3.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected STAT3 in HeLa and A431 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human STAT3.
      EpitopeUnknown
      Clone18F7.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq].
      Gene Symbol
      • APRF
      • FLJ20882
      • HIES
      • MGC16063
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.

      SUBUNIT STRUCTURE: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with NCOA1, PELP1, SOCS7 and STATIP1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts with IL31RA. Interacts with SIPAR. Interacts (via SH2 domain) with NLK By similarity. Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import By similarity. Interacts with TMF1.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation.

      TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

      PTM: Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity.

      INVOLVEMENT IN DISEASE: Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.

      MISCELLANEOUS: Involved in the gp130-mediated signaling pathway.

      SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family.

      Contains 1 SH2 domain.
      Molecular Weight~88 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected STAT3 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-1078 04053252680809

      Documentation

      Anti-STAT3 Antibody, clone 18F7.1 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-STAT3 Antibody, clone 18F7.1 Certificates of Analysis

      TitleLot Number
      Anti-STAT3, clone 18F7.1 - 2278547 2278547
      Anti-STAT3, clone 18F7.1 - 2360021 2360021
      Anti-STAT3, clone 18F7.1 - 3307759 3307759
      Anti-STAT3, clone 18F7.1 - 3501004 3501004
      Anti-STAT3, clone 18F7.1 - NG1944098 NG1944098
      Anti-STAT3, clone 18F7.1 - NRG1781529 NRG1781529
      Anti-STAT3, clone 18F7.1 -2664243 2664243
      Anti-STAT3, clone 18F7.1 -2697399 2697399
      Anti-STAT3, clone 18F7.1 -2752573 2752573
      Anti-STAT3, clone 18F7.1 -2801955 2801955
      Show more

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies