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MABE1107 Anti-SMARCA3 (HLTF) Antibody, clone 5-1

Anti-SMARCA3 (HLTF), clone 5-1 , Cat. No. MABE1107, is a highly specific mouse monoclonal antibody that targets HTLF and has been tested in Immunohistochemistry (Paraffin).

Anti-SMARCA3 (HLTF) Antibody, clone 5-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1 UniProt Number: Q14527
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MABE1107
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1107
      DescriptionAnti-SMARCA3 (HLTF) Antibody, clone 5-1
      Alternate Names
      • Helicase-like transcription factor
      • DNA-binding protein/plasminogen activator inhibitor 1 regulator
      • HIP116
      • RING finger protein 80
      • Sucrose nonfermenting protein 2-like 3
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3
      Background InformationHelicase-like transcription factor (UniProt Q14527; also known as DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, RING finger protein 80, Sucrose non-fermenting protein 2-like 3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3) is encoded by the HLTF (also known as HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1) gene (Gene ID 6596) in human. Helicase-like transcription factor (115 kDa) is a SWI/SNF (mating-type switching/sucrose non-fermenting) protein that uses ATP hydrolysis to remodel chromatin in multiple cellular processes. It is shown to regulate transcription by binding to the SV40 enhancer and the promoters Serpin E1 and HIV-1. It is well expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. It was initially characterized for its DNA binding and transcriptional activity, however, later was shown to act as an E3 ubiquitin ligase and was implicated in post-replication DNA repair by polyubiquitination of proliferating cell nuclear antigen (PCNA). The inactivation of HIP116 is shown to induce an elevated level of chromosome breaks and fusions in mouse embryonic fibroblasts after treatment with alkylating agents. HIP116 inactivation in transgenic mice is reported to disrupt cell cycle at the G2/M phase.

      Ref.: Arcolia, V et al. (2014). BMC Cancer 14, 492.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SMARCA3 (HLTF), clone 5-1 , Cat. No. MABE1107, is a highly specific mouse monoclonal antibody that targets HTLF and has been tested in Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected predominantly nuclear HLTF (SMARCA3) immunoreactivity in human cervical cancer tissue section.
      Biological Information
      ImmunogenGST-tagged recombinant human HLTF (SMARCA3) N-terminal fragment.
      Clone5-1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5-1 detected predominantly nuclear HLTF (SMARCA3) immunoreactivity by immunohistochemistry staining. Clone 5-1 epitope lies within an N-terminal region present in both spliced isoforms of human HLTF reported by UniProt (Q14527).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HLTF
      • HIP116A
      • RNF80
      • SMARCA3
      • SNF2L3
      • ZBU1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight113.9/99.92 kDa (human isoform 1/2) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human breast cancer tissue section.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected predominantly nuclear HLTF (SMARCA3) immunoreactivity in human breast cancer tissue section.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1107 04054839132933