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ABS1587 Anti-Ribophorin I/RPN-I Antibody

This Anti-Ribophorin I/RPN-I Antibody is validated for use in Western Blotting for the detection of Ribophorin I.

Anti-Ribophorin I/RPN-I Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Organelle & Cell Markers Gene Symbol: RPN1 UniProt Number: P04843
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ABS1587
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, CaWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1587
      DescriptionAnti-Ribophorin I/RPN-I Antibody
      Alternate Names
      • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
      • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
      • Ribophorin I
      • RPN-I
      • Ribophorin-1
      Background InformationDolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1 (EC 2.4.99.18; UniProt P04843; also known as Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1) is encoded by the RPN1 gene (Gene ID 6184) in human. Ribophorin I is an ER transmembrane protein and a subunit of the mammalian Oligosaccharyltransferase (OST) complex. Ribophorin I binds newly synthesized target proteins and delivers them to the catalytic core of the OST complex. Association of ribophorin I with newly synthesized membrane proteins was observed after the exit of the nascent chain from the Sec61 translocon, and it was unaffected by the N-glycosylation status. Ribophorin I is reported to preferentially interact with misfolded over native ribonuclease A. In addition, Ribophorin I is shown to complex with another ER protein malectin and enhance malectin's ability to prevent the secretion of the folding-defective proteins.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Ribophorin I/RPN-I Antibody is validated for use in Western Blotting for the detection of Ribophorin I.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: An 1:5,000 dilution from a representative lot detected ribophorin I in in 25 µg of HeLa cell lysate (Courtesy of Dr. Christopher V. Nicchitta, Duke University Medical Center, Durham, NC).
      Western Blotting Analysis: A representative lot detected ribophorin I in the alkali-extracted and Triton X-114-selected integral membrane fraction derived from UV-cross-linked canine pancreas rough microsomes (RM) preparation (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
      Western Blotting Analysis: A representative lot detected ribophorin I in both the Brij35-sensitive (BrS) and Brij35-resistant (BrR) fraction of HeLa ER membrfane proteins (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
      Western Blotting Analysis: A representative lot detected ribophorin I associated with the rRNA-free poly(A) messenger ribonucleoproteins (mRNPs) purified by oligo(dT) from UV-cross-linked canine pancreas rough microsomes (RM) preparation (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
      Western Blotting Analysis: A representative lot detected ribophorin I in both the smooth and rough microsomes sub-fractionated from a rat liver microsome preparation (Menon, A.K., et al. (2000). Curr. Biol. 10(5):241-252).
      Western Blotting Analysis: A representative lot detected ribophorin I in canine rough microsome (RM) fractions obtained by sucrose gradient centrifugation of digitonin- or DHPC-solubilized canine RM preparation with or without ribosomes stripping by EDTA treatment (Potter, M.D., and Nicchitta, C.V. (2000). J. Biol. Chem. 275(3):2037-2045).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal sequence of human Ribophorin I/RPN-I.
      EpitopeC-terminus.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      • Rat
      • Canine
      Species Reactivity NoteHuman, Rat, Canine. Predicted to react with Non-human primates, Porcine, and Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RPN1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~68 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in canine pancreas tissue rough microsome lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Ribophorin I/RPN-I in 2.5 µL of canine pancreas tissue rough microsome lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1587 04055977331295