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05-623-AF647
Sigma-AldrichAnti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate
This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.
More>>This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate
Alternate Names
DNA-directed RNA polymerase II subunit RPB1
RNA polymerase II subunit B1
DNA-directed RNA polymerase II subunit A
DNA-directed RNA polymerase III largest subunit
RNA-directed RNA polymerase II subunit RPB1
RNA polymerase II
Background Information
DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
References
Product Information
Format
AlexaFluor®647
Presentation
Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.
Key Applications
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in A431 and NIH/3T3 cells. The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.
Biological Information
Immunogen
KLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Epitope
carboxyl-terminal domain of RNA polymerase II
Clone
CTD4H8
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
This antibody recognizes Phospho- and non-phospho-RNA polymerase II.
Isotype
IgG1
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse. Predicted to react with Yeast, Rat based on 100% sequence homology.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number
GTIN
05-623-AF647
04055977298918
Documentation
Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate MSDS
