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MABE2019-25UL Anti-PrimPol Antibody, clone JUAM71D

Anti-PrimPol, clone JUAM71D , Cat. No. MABE2019, is a rat monoclonal antibody that detects DNA-directed primase/polymerase (PrimPol) and is tested for use in Immunofluorescence and Western Blotting.

MABE2019-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE2019-25UL
      DescriptionAnti-PrimPol Antibody, clone JUAM71D
      Alternate Names
      • DNA-directed primase/polymerase protein
      • hPrimpol1
      • Coiled-coil domain-containing protein 111
      Background InformationDNA-directed primase/polymerase protein (UniProt: Q96LW4; also known as hPrimpol1, Coiled-coil domain-containing protein 111) is encoded by the PRIMPOL (also known as CCDC111) gene (Gene ID: 201973) in human. PrimPol is an enzyme with both DNA primase and DNA polymerase activities and is involved in DNA damage tolerance. It is detected in nuclei and cytosol with a large fraction localized inside the mitochondria. Upon UV irradiation, it is rapidly redirected to chromatin to reprime DNA synthesis. It associates with nuclear chromatin during the G1 and S phases of unperturbed cell cycles. It contains two RPA1-binding motifs (RBM) (aa 513-527 and 548-556) of which RPA1 is shown to be essential for recruitment to chromatin. It binds to the basic cleft of RPA1. The RPA2-binding motif plays a supporting role in RPA1-binding. PrimPol is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase and is able to bypass certain DNA lesions. It displays high capacity to tolerate DNA damage lesions and is able to synthesize DNA primers downstream of lesions and allows DNA replication to continue. It is also shown to realign primers ahead of 'unreadable lesions' thereby skipping the lesion. In vitro, PrimPol is reported to act as a DNA primase, elongating primers that PrimPol itself synthesizes, or as translesion synthesis DNA polymerase that elongates pre-existing primers across lesions. In murine models PrimPol knockout cells display hypersensitivity to DNA interstrand crosslinks-inducing drugs. Two isoforms of PrimPol have been described that are produced by alternative splicing. (Ref.: Diaz-Talavera, I., et al. (2022). Biomolecules. 12(2); 248; Gonzales-Acosta, D., et al. (2021). EMBO J. 40(14); e106355; Guilliam, TA., et al. (2017). Nat. Commun. 8:15222; Garcia-Gomez, S., et al. (2013). Mol. Cell. 52(4); 541-553; Mouron, S., et al. (2013). Nat. Struct. Mol. Biol. 20(12); 1383-1389).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-PrimPol, clone JUAM71D , Cat. No. MABE2019, is a rat monoclonal antibody that detects DNA-directed primase/polymerase (PrimPol) and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected PrimPol in Western Blotting applications (Gonzalez-Acosta, D., et al. (2021). EMBO J ;40(14); e106355).

      Immunofluorescence Analysis: A representative lot detected PrimPol in Immunofluorescence applications (Gonzalez-Acosta, D., et al. (2021). EMBO J. 40(14); e106355).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged, full-length, recombinant human DNA-directed primase/polymerase (PrimPol).
      EpitopeUnknown
      CloneJUAM71D
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone JUAM71D is a rat monoclonal antibody that detects DNA-directed primase/polymerase (PrimPol).
      IsotypeIgG2b
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRIMPOL
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~64 kDa observed; 64.61 kDa calculated Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected PrimPol in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE2019-25UL 04065269800170