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PC10L Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb

Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC10L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~21 kDa H-, K-, and N-Ras proteins containing aspartic acid at position 12.
      Catalogue NumberPC10L
      Brand Family Calbiochem®
      References
      ReferencesBos, J.L., et al. 1988. Mutation Res. 195, 255.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1990 Nature 287, 686.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate, 100 µg BSA.
      Negative controlSW480 cells
      Positive controlHS578T cells or purified recombinant K-Ras Asp¹² protein
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-5 µg/ml)
      Application CommentsDoes not react with rasGly-12 or rasVal-12 p21's. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (KLVVVGADGVGK) corresponding to amino acids 5-16 of Ras (H, K, or N) with Asp at position 12
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage with 0.1% sodium azide. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC10L 0

      Documentation

      Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC10L

      References

      Reference overview
      Bos, J.L., et al. 1988. Mutation Res. 195, 255.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1990 Nature 287, 686.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-June-2011 RFH
      ApplicationImmunoblotting (1-5 µg/ml)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~21 kDa H-, K-, and N-Ras proteins.
      BackgroundThe human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H-and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and its relevance to human disease diagnosis and prognosis remains to be determined.
      HostRabbit
      Immunogena synthetic peptide (KLVVVGADGVGK) corresponding to amino acids 5-16 of Ras (H, K, or N) with Asp at position 12
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlHS578T cells or purified recombinant K-Ras Asp¹² protein
      Negative controlSW480 cells
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate, 100 µg BSA.
      PreservativeNone
      CommentsDoes not react with rasGly-12 or rasVal-12 p21's. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage with 0.1% sodium azide. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesBos, J.L., et al. 1988. Mutation Res. 195, 255.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1990 Nature 287, 686.