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AB9566-I Anti-OTX2 Antibody

Anti-OTX2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: OTX2 UniProt Number: P32243
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AB9566-I
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9566-I
      DescriptionAnti-OTX2 Antibody
      Alternate Names
      • OTX2
      • Homeobox protein OTX2
      • Orthodenticle homolog 2
      Background InformationHomeobox protein OTX2 (UniProt P32243; also known as Orthodenticle homolog 2) is encoded by the OTX2 (also known as CPHD6, MCOPS5) gene (Gene ID 5015) in human. OTX2 is a paired-type homeobox transcription factor that controls the expression of target genes by binding to regulatory regions containing the YTAATNN motifs. Studies conducted in mice shows developmental stage-dependent expression pattern. OTX2 is widely expressed in the epiblast, but becomes progressively regionalised during gastrulation to an anterior domain of the ectoderm (anterior neuroectoderm, ANE) that contains the precursor cells of the prosencephalon and the mesencephalon of the embryonic brain. The precursor cells for the anterior visceral endoderm (AVE) fail to translocate from the distal site to the anterior region of the pre-gastrulation stage Otx2-knockout embryos. The absence of the AVE, in turn, causes the loss of the source of morphogenetic signals for inducing neural differentiation of the ANE. In addtion, OTX2 is shown to be essential in the anterior mesendoderm (AME) for the induction of anterior neural tissues and head formation. OTX2 is reported to mediate the transcriptions of Dkk1 and Lhx1, loss of Otx2 in the AME results in the downregulation of Dkk1 and Lhx1.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected OTX2 in human retina tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the internal region of human OTX2.
      EpitopeInternal region
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to react with both spliced isoforms, as well as all five polymorphic forms of human OTX2 reported by UniProt (P32243). The immunogen sequence is about 78% conserved in OTX1, this polyclonal antibody may therefore recognize OTX1. However, OTX1 cross-reactivity has not been directly tested and confirmed. OTX2 is expressed earlier than OTX1 and therefore this antibody can be used to identify OTX2 during development.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Rhesus Macaque, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OTX2
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~35 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Y79 human retinoblastoma cell lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected OTX2 in 10 µg of Y79 human retinoblastoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB9566-I 04055977311778

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies