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MABS1283 Anti-MLN64/STARD3 Antibody, clone 2BE-2F4

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MABS1283
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHC, EMMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABS1283
      DescriptionAnti-MLN64/STARD3 Antibody, clone 2BE-2F4
      Alternate Names
      • StAR-related lipid transfer protein 3
      • MLN64/STARD3
      • Metastatic lymph node gene 64 protein
      • MLN 64
      • Protein CAB1
      • START domain-containing protein 3
      • StARD3
      Background InformationMLN64/StARD3 (also known as es64, StAR-related lipid transfer protein 3, Metastatic lymph node gene 64 protein, Metastatic lymph node protein 64, StARD3, START domain-containing protein 3, Steroidogenic acute regulatory protein related; UniProt Q14849) is encoded by the MLN64 (or CAB1 & STARD3) gene in human (NCBI Gene ID 10948). MLN64/StARD3 is a late endosome (LE) multitransmembrane protein that plays an important role in intracellular cholesterol transport and steroidogenesis. In addition to binding cholesterol via its C-terminal STAR domain, MLN64/StARD3 is reported to interact with ER-anchored VAP proteins via a conserved motif called "two phenylalanines in an acidic tract" or FFAT, thereby forming an LE-ER tethering complex at the inter-organelle membrane contacts sites (MCSs), allowing heterologous membrane apposition and the exchange of metabolites (PMID 23709693 and 24105263). Clone 2BE-2F4 is also known as 2BE2F4 and mAbMLN64-Ct, it detects an epitope within the c-terminal region encompassing the last 20% of MLN64 sequence.
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1κ ascites with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MLN64/STARD3 Antibody, clone 2BE-2F4 is an antibody against MLN64/STARD3 for use in Western Blotting and Immunocytochemistry, Immunohistochemistry, Electron Microscopy.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Electron Microscopy
      Application NotesImmunocytochemistry Analysis: A representative lot detected MLN64/STARD3 in MCF7/MLN64 cells (Alpy, F., et al. (2001). J. Biol. Chem. 276:4261-4269).
      Immunocytochemistry Analysis: A representative lot detected MLN64/STARD3 in BT-474, MCF7, and transfected COS-1 cells (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).
      Immunohistochemistry Analysis: A representative lot detected MLN64/STARD3 in human breast tissue (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).
      Electron Microscopy Analysis: A representative lot detected MLN64/STARD3 in HeLa cells (Holtta-Vuori, M., et al. (2005). Molecular Biology of the Cell. 16:3873-3886).
      Western Blotting Analysis: A representative lot detected MLN64/STARD3 in COS-1 cell lysate (Watari, H., et al. (1997). Proc. Natl. Acad. Sci. USA. 94:8462-8467).
      Western Blotting Analysis: A representative lot detected MLN64/STARD3 in various human cell lines (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to human MLN64/STARD3 near the C-terminus.
      EpitopeNear C-terminus
      Clone2BE-2F4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • STARD3
      • CAB1
      • MLN64
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~50 kDa observed. This protein has isoforms at ~48 and ~49 kDa, which are covered by this antigen.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SK-OV-3 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected MLN64/STARD3 in 10 µg of SK-OV-3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1283 04055977162738