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OP134 Anti-IKKβ Mouse mAb (10AG2)

This Anti-IKKβ Mouse mAb (10AG2) is validated for use in Immunoblotting for the detection of IKKβ.

Anti-IKKβ Mouse mAb (10AG2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP134
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~87 kDa IKKβ protein in Daudi cells.
      Catalogue NumberOP134
      Brand Family Calbiochem®
      Application Data
      Detection of human IKKβ by immunoblotting. Sample: Whole cell lysate from Daudi cells (30 µg). Primary antibody: Anti-IKKβ Mouse mAb (10AG2) (Cat. No. OP134) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesDelhase, M., et al. 1999. Science 284, 309.
      Li. Q., et al. 1999. Science 284, 321.
      Didonato, et al. 1997. Nature 388, 548.
      Lee, F.S., et al. 1997. Cell 88, 213.
      Malinin, N.L., et al. 1997. Nature 385, 540.
      Mercurio, F., et al. 1997. Science 278, 860.
      Regnier, C.H., et al. 1997. Cell 90, 373.
      Verma, I. And Stevenson, J.K. 1997. Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995. Genes. Dev. 9, 2723.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.2% gelatin, pH 7.4.
      Positive controlDaudi cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-4 µg/ml, chemiluminescence)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of full-length human IKKβ fused to a His•Tag® sequence
      ImmunogenHuman
      Clone10AG2
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP134 0

      Documentation

      Anti-IKKβ Mouse mAb (10AG2) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-IKKβ Mouse mAb (10AG2) Certificates of Analysis

      TitleLot Number
      OP134

      References

      Reference overview
      Delhase, M., et al. 1999. Science 284, 309.
      Li. Q., et al. 1999. Science 284, 321.
      Didonato, et al. 1997. Nature 388, 548.
      Lee, F.S., et al. 1997. Cell 88, 213.
      Malinin, N.L., et al. 1997. Nature 385, 540.
      Mercurio, F., et al. 1997. Science 278, 860.
      Regnier, C.H., et al. 1997. Cell 90, 373.
      Verma, I. And Stevenson, J.K. 1997. Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995. Genes. Dev. 9, 2723.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationImmunoblotting (1-4 µg/ml, chemiluminescence)
      Application Data
      Detection of human IKKβ by immunoblotting. Sample: Whole cell lysate from Daudi cells (30 µg). Primary antibody: Anti-IKKβ Mouse mAb (10AG2) (Cat. No. OP134) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes the ~87 kDa IKKβ protein.
      BackgroundNF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/γ. IKK1/α and IKK2/β are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/β not IKK1/α, is the target proinflammatory stimuli. Targeted disruption of IKK2/β gene in mice also results in extensive liver damage from apotopsis and death as embryo.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of full-length human IKKβ fused to a His•Tag® sequence
      Clone10AG2
      IsotypeIgG₁
      Specieshuman
      Positive controlDaudi cells
      FormLiquid
      FormulationIn PBS, 0.2% gelatin, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDelhase, M., et al. 1999. Science 284, 309.
      Li. Q., et al. 1999. Science 284, 321.
      Didonato, et al. 1997. Nature 388, 548.
      Lee, F.S., et al. 1997. Cell 88, 213.
      Malinin, N.L., et al. 1997. Nature 385, 540.
      Mercurio, F., et al. 1997. Science 278, 860.
      Regnier, C.H., et al. 1997. Cell 90, 373.
      Verma, I. And Stevenson, J.K. 1997. Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995. Genes. Dev. 9, 2723.