Millipore Sigma Vibrant Logo

ABE76-I Anti-Enterobacterio Phage MS2 Coat Protein

Anti-EnterobacterioPhage MS2 Coat Protein, Cat. No. ABE76-I, is a highly specific rabbit polyclonal antibody that targets Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein and has been tested in Immunoprecipitation and Western Blotting.

Anti-Enterobacterio Phage MS2 Coat Protein MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function UniProt Number: P03612
View Products on Sigmaaldrich.com
ABE76-I
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AIP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE76-I
      DescriptionAnti-Enterobacterio Phage MS2 Coat Protein
      Alternate Names
      • Capsid protein
      • CP
      • Coat protein
      • MS2 Coat Protein
      • Enterobacterio Phage MS2 Coat Protein
      Background InformationThe bacteriophage MS2 is a small, male-specific RNA bacteriophage that infects E. coli and other members of the Enterobacteriaceae family. Its genome comprises a single sense (+) strand of RNA 3569 nucleotides in length, which encodes only four proteins: the coat protein, the A or maturation protein, which is required for infectivity, a subunit of the viral replicase, and a small lysis protein encoded in an overlapping reading frame. The capsid protein (UniProt: P03612; also known as CP, Coat protein) of bacteriophage MS2 is a translational repressor. It is reported to inhibit the synthesis of the viral replicase by binding a specific RNA structure that contains the replicase translation initiation region. Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry and consists of 89 capsid proteins dimers (178 capsid proteins). It is involved in viral genome encapsidation through the interaction between a capsid protein homodimer and the multiple packaging signals present in the RNA genome. Proline 78 is shown to be essential for viral infectivity. (Ref.: Hill, HR et al. (1997). J. Mol. Biol. 266(1); 1-7).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-EnterobacterioPhage MS2 Coat Protein, Cat. No. ABE76-I, is a highly specific rabbit polyclonal antibody that targets Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein and has been tested in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:5,000 dilution from a representative lot detected recombinant EnterobacterioPhage MS2 Coat Protein and wildtype MS2 protein (Courtesy of Caroline Horizny from CNSE at SUNY Albany, Albany, NY).

      Immunoprecipitation Analysis: A 1:10,000 dilution from a representative lot detected EnterobacterioPhage MS2 Coat Protein in K562 cells treated with Escherichia phage MS2 (Bacteriophage MS2).
      Biological Information
      ImmunogenHis-tagged recombinant Enterobacteriophage MS2 Coat Protein (aa 2-130).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects MS2 bacteriophage capsid protein. The immunogen used has an N- terminus His tag and mutations to avoid dimerization and capsid formation.
      Species Reactivity
      • All
      Species Reactivity NoteVirus.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~14 kDa observed; 13.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 treated with Escherichia phage MS2 (Bacteriophage MS2) and K562+MS2 recombinant protein treated with Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected EnterobacterioPhage MS2 Coat Protein in K562 ells treated with Escherichia phage MS2 (Bacteriophage MS2) and K562+MS2 recombinant protein treated with Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE76-I 04054839361036

      Documentation

      Anti-Enterobacterio Phage MS2 Coat Protein MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Enterobacterio Phage MS2 Coat Protein Certificates of Analysis

      TitleLot Number
      Anti-Enterobacterio Phage MS2 Coat Protein 3077873
      Anti-Enterobacterio Phage MS2 Coat Protein - 3439355 3439355
      Anti-Enterobacterio Phage MS2 Coat Protein - 3764751 3764751
      Anti-Enterobacterio Phage MS2 Coat Protein - 4051795 4051795
      Anti-Enterobacterio Phage MS2 Coat Protein - 4093610 4093610
      Anti-Enterobacterio Phage MS2 Coat Protein - 4184515 4184515
      Anti-Enterobacterio Phage MS2 Coat Protein - 4232904 4232904
      Anti-Enterobacterio Phage MS2 Coat Protein Polyclonal Antibody 2988804
      Anti-Enterobacterio Phage MS2 Coat Protein Polyclonal Antibody Q2989795
      Anti-Enterobacterio Phage MS2 Coat Protein Polyclonal Antibody 3126016
      Show more