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205601 Anti-E-Cadherin Mouse mAb (HECD-1)

205601
  
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      Overview

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.



      We are offering Anti-E-cadherin Antibody, clone DECMA-1 (Cat. No. MABT26) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






      Recognizes the E-cadherin protein.
      Catalogue Number205601
      Brand Family Calbiochem®
      References
      ReferencesJankowski, J.A., et al. 1994. Int. J. Oncol. 4, 441.
      Rasbridge, S.A., et al. 1993. J. Pathol. 169, 245.
      Tohma, Y., et al. 1992. Cancer Res. 52, 1981.
      Shimoyama, Y., and Hirohashi, S. 1991. Cancer Res. 51, 2185.
      Shiozaki, H., et al. 1991. Am. J. Pathol. 139, 17.
      Takeichi, M. 1991. Science 251, 1451.
      Shimoyama, Y., et al. 1989. Cancer Res. 49, 2128.
      Product Information
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
      Positive controlMCF-7 cells
      PreservativeNone
      Applications
      Key Applications Cell Inhibition Assays
      Competition Studies
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunohistochemistry
      Application NotesCompetition Studies (100 μg/ml)
      ELISA (0.2-1 μg/ml)
      Flow Cytometry (1 μg/ml)
      Immunoblotting (2-10 μg/ml)
      Immunofluorescence (1 μg/ml)
      Immunohistochemistry (2-10 μg/ml)
      Application CommentsMay be used to inhibit E-cadherin-dependent cell-cell contact. Variables associated with assay conditions will dictate the optimal dilution.
      Biological Information
      ImmunogenMCF-7 human breast carcinoma cell line
      ImmunogenHuman
      CloneHECD-1
      HostMouse
      IsotypeIgG₁
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 50 μl dH₂O final concentration 2 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Note: Ca2+ should be present in all buffers to stabilize the cadherin antigen.
      Global Trade Item Number
      Catalogue Number GTIN
      205601 0