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219010 Anti-Catalase Rabbit pAb

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

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219010-1ML
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      Description
      OverviewRecognizes the ~60-65 kDa catalase protein.
      Catalogue Number219010
      Brand Family Calbiochem®
      Application Data
      Detection of human catalase by immunoblotting. Samples: Low molecular weight markers (lanes 1, 5a, 5b, 9), catalase protein (0.075 µg) (lanes 2 and 6), catalase protein (0.15 µg) (lanes 3 and 7), and catalase protein (0.3 µg) (lanes 4 and 8), and red blood cell lysate (lane 10). With primary antibody dilution 1:8000 (lanes 1-5a) and 1:6000 (lanes 5b-10). Primary antibody: Anti-Catalase Rabbit pAb (Cat. No. 219010) (1:6000). Detection: chemiluminescence.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlRed blood cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Paraffin Sections Meilhac, O., et al. 2001. Arterioscler. Thromb. Vasc. Biol. 21, 1681.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoelectrophoresis
      Application NotesIndirect ELISA (1:128)
      Immunoblotting (1:8000; see application references)
      Immunoelectrophoresis (see comments)
      Paraffin Sections (see application references)
      Application CommentsThe 256 kDa catalase protein is a tetramer of 4 identical ~60-65 kDa protein. Exhibits a single arc by immunoelectrophoresis (IEP) vs. human red cell lysate. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified, human erythrocytes catalase
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      219010-1ML 04055977202045