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AP1043 Anti-Caspase-1 Mouse mAb (14F468)

Anti-Caspase-1 Mouse mAb (14F468) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme

AP1043
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~45 kDa latent form of caspase-1 in HeLa and NIH3T3 cells. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390.
      Catalogue NumberAP1043
      Brand Family Calbiochem®
      SynonymsAnti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme
      Application Data
      Detection of human and mouse caspase-1 by immunoblotting. Samples: Whole cell lysate from HeLa (lane A) and NIH3T3 cells (lane B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043) (0.1 µg/ml, HeLa lysate and 2 µg/ml, NIH3T3 lysate). Detection: chemiluminescence.

      Detection of human caspase-1 by staining paraffin sections. Sample: Colon carcinoma, formalin-fixed (panels A and B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043). Detection: DAB with hematoxylin counterstain.
      References
      ReferencesTatsuta, T., et al. 2000. J. Biol. Chem. 275, 14248.
      Li, P., et al. 1995. Cell 80, 401.
      Yuan, J., et al. 1993. Cell 75, 641.
      Cerretti, D.P., et al. 1992. Science 256, 97.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHeLa and NIH 3T3 cells and colon carcinoma
      Preservative≤ 0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (0.5-2 µg/ml)
      Paraffin Sections (see comments)
      Application CommentsWill stain formalin-fixed, paraffin-embedded tissues, but requires titration for individual samples. The immunogen sequence is also conserved in rat caspase-1, but cross-reactivity has not been tested. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (RKVRFSFEQPDGRAQMPTTE) corresponding to amino acids 371-390 of human caspase-1
      ImmunogenHuman
      Clone14F468
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1043 0

      Documentation

      Anti-Caspase-1 Mouse mAb (14F468) Certificates of Analysis

      TitleLot Number
      AP1043

      References

      Reference overview
      Tatsuta, T., et al. 2000. J. Biol. Chem. 275, 14248.
      Li, P., et al. 1995. Cell 80, 401.
      Yuan, J., et al. 1993. Cell 75, 641.
      Cerretti, D.P., et al. 1992. Science 256, 97.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme
      ApplicationImmunoblotting (0.5-2 µg/ml)
      Paraffin Sections (see comments)
      Application Data
      Detection of human and mouse caspase-1 by immunoblotting. Samples: Whole cell lysate from HeLa (lane A) and NIH3T3 cells (lane B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043) (0.1 µg/ml, HeLa lysate and 2 µg/ml, NIH3T3 lysate). Detection: chemiluminescence.

      Detection of human caspase-1 by staining paraffin sections. Sample: Colon carcinoma, formalin-fixed (panels A and B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043). Detection: DAB with hematoxylin counterstain.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~45 kDa latent form of caspase-1.
      BackgroundCaspases are a family of cysteine proteases that play a key role in mediating apoptosis. All caspases are produced as inactive enzymes that require cleavage of a prodomain, followed by a second cleavage to generate the large and small subunits that form the tetrameric, active enzyme. Caspase-1, also known as ICE (IL-1β converting enzyme), was first isolated from monocytic lineage cells, and was so named because it converts the 33 kDa pro-form of IL-1β to the active, 17.5 kDa form via a specific, tightly controlled proteolytic cleavage process.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (RKVRFSFEQPDGRAQMPTTE) corresponding to amino acids 371-390 of human caspase-1
      Clone14F468
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlHeLa and NIH 3T3 cells and colon carcinoma
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤ 0.1% sodium azide
      CommentsWill stain formalin-fixed, paraffin-embedded tissues, but requires titration for individual samples. The immunogen sequence is also conserved in rat caspase-1, but cross-reactivity has not been tested. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesTatsuta, T., et al. 2000. J. Biol. Chem. 275, 14248.
      Li, P., et al. 1995. Cell 80, 401.
      Yuan, J., et al. 1993. Cell 75, 641.
      Cerretti, D.P., et al. 1992. Science 256, 97.