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ABN182 Anti-CYP27B1 Antibody

This Anti-CYP27B1 Antibody is validated for use in Western Blotting, IHC for the detection of CYP27B1.

Anti-CYP27B1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: CYP27B1, CYP1ALPHA, CYP27B UniProt Number: O15528
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ABN182
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN182
      DescriptionAnti-CYP27B1 Antibody
      Alternate Names
      • 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
      • 25-OHD-1 alpha-hydroxylase
      • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
      • VD3 1A hydroxylase
      • Calcidiol 1-monooxygenase
      • Cytochrome P450 subfamily XXVIIB polypeptide 1
      • Cytochrome P450C1 alpha
      • Cytochrome P450VD1-alpha
      • Cytochrome p450 27B1
      Background Information25-hydroxyvitamin D-1 alpha hydroxylase (1αOHase; VD3 1A hydroxylase; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; or CYP27B1) is a mitochondrial enzyme that belongs to the cytochrome P450 family. It catalyzes the hydroxylation of 25-hydroxyvitamin D3 to form an active 1-alpha, 25-dihydroxyvitamin D3, in the kidneys and other tissues. 25-hydroxyvitamin D-1 alpha hydroxylase plays an important role in bone growth, calcium metabolism, and tissue differentiation. Previous studies have reported that mice deficient in 25-hydroxyvitamin D-1 alpha hydroxylase are affected by hypocalcemia, secondary hyperparathyroidism, retarded growth, and the skeletal abnormalities. In humans, defects in this enzyme causes pseudovitamin D deficiency rickets (vitamin D-dependent type 1A rickets), an autosomal recessive condition characterized by abnormal bone mineralization and impaired growth.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Serum free MCF-7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-CYP27B1 Antibody is validated for use in Western Blotting, IHC for the detection of CYP27B1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CYP27B1 in mouse and rat hippocampus tissue and in rat kidney tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human CYP27B1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Predicted to react with Chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CYP27B1
      • CYP1ALPHA
      • CYP27B
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~58 kDa observed. Uncharacterized bands may be observed at ~55 kDa, ~70 kDa, ~110 kDa, and ~115 kDa in some cell lysates. A doublet at ~58 kDa may be observed in some cell lysates (Kemmis, C. M., et al. (2006). J Nutr. 136(4):887-892.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in serum free MCF-7 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected CYP27B1 in 10 µg of serum free MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN182 04053252696770

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies