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217570 Anti-CD3 Mouse mAb (UCHT1)

Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Anti-CD3 Mouse mAb (UCHT1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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217570
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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217570-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.
      Catalogue Number217570
      Brand Family Calbiochem®
      References
      ReferencesThibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
      Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Preservative0.5 mg/ml gentamycin sulfate
      Quality LevelMQ100
      Applications
      Application ReferencesT cell activation Lafont, V., et al. 1999. J. Biol. Chem. 274, 25743.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoprecipitation
      T Cell Co-stimulation
      Application NotesFlow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      T cell Co-stimulation (see application references)
      Application CommentsThis antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman thymocytes/Sezary T cells
      ImmunogenHuman
      CloneUCHT1
      HostMouse
      Purity>95% immunoglobulin by SDS-PAGE
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      217570-100UG 04055977219418

      Documentation

      Anti-CD3 Mouse mAb (UCHT1) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD3 Mouse mAb (UCHT1) Certificates of Analysis

      TitleLot Number
      217570

      References

      Reference overview
      Thibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
      Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.

      Citations

      Title
    • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      ApplicationFlow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      T cell Co-stimulation (see application references)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman thymocytes/Sezary T cells
      CloneUCHT1
      IsotypeIgG₁
      Specieshuman
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity>95% immunoglobulin by SDS-PAGE
      Preservative0.5 mg/ml gentamycin sulfate
      CommentsThis antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesThibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
      Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.
      Citation
    • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
      		•
      Application referencesT cell activation Lafont, V., et al. 1999. J. Biol. Chem. 274, 25743.