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MABC573 Anti-Bcl-2 Antibody, clone 1D7.2

Detect Bcl-2 using this rat Monoclonal Anti-Bcl-2 Antibody, clone 1D7.2, Cat. No. MABC573, validated for use in Western Blotting.

Anti-Bcl-2 Antibody, clone 1D7.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: Bcl2, Bcl-2 UniProt Number: P10417
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MABC573
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC573
      DescriptionAnti-Bcl-2 Antibody, clone 1D7.2
      Alternate Names
      • Apoptosis regulator Bcl-2
      • Bcl-2
      Background InformationApoptosis regulator Bcl-2 (UniProt P10417; also known as B-cell leukemia/lymphoma 2) is encoded by the Bcl2 (also known as Bcl-2, AW986256, C430015F12Rik, D630044D05Rik, D830018M01Rik) gene (Gene ID 12043) in murine species. Bcl-2 is an anti-apoptotic and the founding member of the Bcl-2 family of proteins that paly either a pro- or anti-apoptotic role in cell death signaling. BCL-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four BCL-2 homology regions (BH1–BH4), whereas the BH3-only members contain only the BH3 amphipathic helix, which mediates their interaction with the groove of multi-domain BCL-2 family members. Human BCL2 gene mutatons are well known causes of many cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Bcl-2 using this rat Monoclonal Anti-Bcl-2 Antibody, clone 1D7.2, Cat. No. MABC573, validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminal of mouse Bcl-2.
      EpitopeN-terminal half
      Clone1D7.2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityExpected to react with both spliced isoforms of murine Bcl-2.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Bcl2
      • Bcl-2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~22 kDa observed (26.4 kDa/isoform 1 & 22.3 kDa/isoform 2, calculated).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse myoblast C2C12 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Bcl-2 in 10 µg of mouse myoblast C2C12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC573 04055977176384