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Merck

IPVH07850

Immobilon® -P PVDF Membrane

50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, mini blots, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22

Key Documents

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Product Name

Immobilon®-P PVDF Membrane, 50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane
plain filter
white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
 215 μg/cm2 adsorption capacity (BSA)
 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

Quality Level

400

Related Categories

Application

Immobilon®-P PVDF Membrane has been used in western blotting.

Features and Benefits

Immobilon®-P membrane has:
  • excellent protein retention
  • high physical strength
  • broad chemical compatibility
  • suitable for a variety of staining applications and reprobing in immunodetection methods

General description

Immobilon®-P transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (μm). It is recommended for western blotting, especially for proteins greater than 20 kDa. Immobilon®-P transfer membrane can bind a wide range of molecular weight proteins. It is used for the transfer of proteins from a variety of gel matrices. When compared to a nitrocellulose membrane, the Immobilon-P PVDF membrane has a higher signal-to-noise ratio for enhanced sensitivities, improved handling characteristics and staining capabilities and increased solvent resistance.
Primary Binding Mechanisms: Electrostatic, hydrophobic;
Filter Code: IPVH

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rong Kang et al.
American journal of otolaryngology, 33(6), 678-684 (2012-07-10)
The purposes of this study were to determine whether autophagy was involved in cisplatin (CDDP) resistance and to investigate the role of the autophagy in the regulation of chemosensitivity to CDDP in laryngeal cancer Hep-2 cells. A WST-1 assay was
Paola Maycotte et al.
Autophagy, 8(2), 200-212 (2012-01-19)
Chloroquine (CQ) is a 4-aminoquinoline drug used for the treatment of diverse diseases. It inhibits lysosomal acidification and therefore prevents autophagy by blocking autophagosome fusion and degradation. In cancer treatment, CQ is often used in combination with chemotherapeutic drugs and
Sheikh F Ahmad et al.
Neurotoxicology, 77, 1-11 (2019-12-08)
Autism spectrum disorder (ASD) comprises a broad range of neurodevelopmental disorders that are associated with deficits in social interaction and communication. The tyrosine kinase inhibitor tyrphostin AG126 represents a promising therapeutic agent for several neuroinflammatory disorders. There are currently no
Kentaro Togashi et al.
Journal of the neurological sciences, 413, 116802-116802 (2020-04-04)
Parkinson's disease (PD) is characterized by progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc). Levodopa (L-Dopa), the current main treatment for PD, reduces PD symptoms by partially replacing dopamine, but it does not slow neurodegeneration. Recent

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Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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Global Trade Item Number

SKUGTIN
IPVH0785004053252675676

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