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Merck

480708

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

Synonym(s):

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase

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About This Item

CAS Number:
9001-67-6
EC Number:
3.2.1.18 (BRENDA, IUBMB)
NACRES:
NA.54
UNSPSC Code:
12352202
MDL number:
MFCD00149915

Key Documents

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recombinant

expressed in E. coli

description

RTECS - QQ3450000

product line

EMPROVE® EXPERT

form

liquid

specific activity

≥10 units/mL
≥250 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Quality Level

100

Related Categories

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Disclaimer

Toxicity: Harmful (C)

General description

Note: 1 mU = 1 milliunit.
Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

8B - Non-combustible, corrosive hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yi Yang et al.
International journal of molecular sciences, 21(22) (2020-11-19)
Polysialic acid (polySia/PSA) is a linear homopolymer of sialic acid (Sia) that primarily modifies the neural cell adhesion molecule (NCAM) in mammalian brains. PolySia-NCAM not only displays an anti-adhesive function due to the hydration effect, but also possesses a molecule-retaining
Airi Mori et al.
International journal of molecular sciences, 21(16) (2020-08-23)
Polysialic acid (polySia/PSA) is an anionic glycan polymer of sialic acid, and it mostly modifies the neural cell adhesion molecule (NCAM) in mammalian brains. Quality and quantity of the polySia of the polySia-NCAM is spatio-temporally regulated in normal brain development

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