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407207 SignalBoost™Immunoreaction Enhancer Kit

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407207
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Overview

Key Spec Table

FormatDetection Methods
Liquid, Antibody DiluentChemiluminescence, colorimetric, or fluorescence

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407207-1KIT
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      Description
      OverviewSignalBoost™ Immunoreaction Enhancer Kit is designed to enhance the desired signal in assays such as immunoblotting, dot blotting, and ELISA. This kit significantly improves sensitivity and discrimination when assessing protein targets of low immunoreactivity or low expression levels. Enhancement of signal is especially marked for low affinity antibodies. It replaces conventional antibody diluents with Solution 1, which is used to dilute primary antibodies and Solution 2, which is used to dilute secondary antibodies. No additional steps, materials, or increase of assay time is required.

      Increases signal: minimizes background resulting in high signal to noise ratios
      Versatile: can be used with nitrocellulose or PVDF membranes and is compatible with chemiluminescent or colorimetric detection systems
      Convenient: ready-to-use format, no dilution necessary
      Catalogue Number407207
      Brand Family Calbiochem®
      Application Data
      Detection of protein kinase C by immunoblotting. Samples: whole cell lysate from K562 cells. Immunoblotting conditions: nitrocellulose membrane blocked overnight with 5% milk in PBST; all washes were performed with PBST. Primary antibody: Anti-PKC (Ab-2) Mouse mAb (MC5) (Cat. No. OP74) diluted 1:1000 in 5% milk/PBST (lane A) or in Solution 1 (lane B) (incubation for 1 h ). Secondary antibody: Anti-Mouse IgG diluted 1:10,000 in 5% milk/PBST (lane A) or Solution 2 (lane B). Detection: chemiluminescence (30 s exposure).

      ELISA results generated with and without dilution of antigen and detection antibody using SignalBoost™ reagent.
      Materials Required but Not Delivered Any equipment necessary for performing the desired immunoassay
      Primary antibody
      Secondary antibody (labeled with HRP, AP, etc.) specific to the primary antibody
      Product Information
      Detection methodChemiluminescence, colorimetric, or fluorescence
      Form20 Miniblots or 1600 cm²
      FormatLiquid, Antibody Diluent
      Kit containsSolution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol.
      Product Usage Statements
      Intended useSignalBoost™ Immunoreaction Enhancer Solution is a reactive reagent for use in immunoassays, and was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as immunoblotting and ELISA (enzyme-linked immunosorbent assay). It can be used in a range of immunoassays.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Storage ConditionsUpon receipt, store the entire contents of the kit at 4°C. Protect from light. Following initial use, this product is stable for up to 1 year at 4°C.
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsSolution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      407207-1KIT 07790788050023