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AP1062 PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb

This PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb is validated for use in Immunoblotting, IP, ICC for the detection of PDH-E1α (pSer²⁹³).

PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb

AP1062
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, Pm, RRbPolyclonal Antibody

Pricing & Availability

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AP1062-50UL
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      AP1062-5MG
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      In Stock 
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      Limited Quantities Available
      Availability to be confirmed
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          Description
          OverviewRecognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
          Catalogue NumberAP1062
          Brand Family Calbiochem®
          SynonymsAnti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb
          Application Data
          Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).

          Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.

          Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.
          References
          ReferencesSeifert, F., et al. 2007. Biochemistry 21, 6277.
          Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
          Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
          Positive controlHEK293 cells
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (1:10,000)
          Immunoprecipitation (1:50)
          Immunocytochemistry (1:500)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Primate
          • Rat
          Antibody TypePolyclonal Antibody
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Global Trade Item Number
          Catalogue Number GTIN
          AP1062-50UL 04055977227765
          AP1062-5MG 04053252046513