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341292 Sigma-AldrichPhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb

This PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr³⁹⁷).

PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

CoA
References
Data Sheet

Synonyms: Anti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)

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Overview

Replacement Information

Key Spec Table

Species Reactivity	Host	Antibody Type
Ch, Dr, Fg, H, M, R	Rb	Polyclonal Antibody

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Description
Overview	Recognizes the ~125 kDa FAK protein when phosphorylated at Tyr³⁹⁷. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number	341292
Brand Family	Calbiochem®
Synonyms	Anti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
Application Data	Detection of FAK, phosphorylated on Tyr³⁹⁷, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.

References
References	Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235. Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250. Vial, D., et al. 2000. J. Biol. Chem. 275, 28269. Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758. Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.

Product Information
Form	Liquid
Formulation	In Dulbecco's PBS (no Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Positive control	Chicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunohistochemistry
Application Notes	Immunoblotting (1:1000) Immunohistochemistry (see comments; heat pre-treatment required)
Application Comments	Although the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
Host	Rabbit
Isotype	IgG
Species Reactivity	Chicken Drosophila Frog Human Mouse Rat
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
341292-10T	04055977215625

Documentation

PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb Certificates of Analysis

Title	Lot Number
341292	Enter Lot Number

References

Reference overview
Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235. Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250. Vial, D., et al. 2000. J. Biol. Chem. 275, 28269. Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758. Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	19-November-2008 JSW
Synonyms	Anti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
Application	Immunoblotting (1:1000) Immunohistochemistry (see comments; heat pre-treatment required)
Application Data	Detection of FAK, phosphorylated on Tyr³⁹⁷, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
Description	Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated on Tyr³⁹⁷.
Background	Discovered as a substrate for Src, FAK acts as a key element of integrin signaling. FAK is a non-receptor tyrosine kinase that plays a central role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. The Tyr³⁹⁷ autophosphorylation site binds Src family SH2 and the p85 subunit of PI3-Kinase.
Host	Rabbit
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
Isotype	IgG
Species	Drosophila, chicken, frog, human, mouse, rat
Positive control	Chicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
Form	Liquid
Formulation	In Dulbecco's PBS (no Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Preservative	≤0.1% sodium azide
Comments	Although the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw ≤ -70°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235. Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250. Vial, D., et al. 2000. J. Biol. Chem. 275, 28269. Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758. Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.

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