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482732 Nitric Oxide Synthase, Constitutive, Bovine Endothelial, Recombinant, S. frugiperda

Nitric Oxide Synthase, Constitutive, Bovine Endothelial, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: eNOS, NOS III, Endothelial Nitric Oxide Synthase

482732
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant bovine endothelial NOS expressed in S. frugiperda insect cells using a baculovirus expression system. eNOS undergoes several post-translational modifications, including myristoylation and palmitoylation, which causes the enzyme to become membrane-associated.
      Catalogue Number482732
      Brand Family Calbiochem®
      SynonymseNOS, NOS III, Endothelial Nitric Oxide Synthase
      References
      ReferencesBusconi, L., and Michel, T. 1995. Mol. Pharmacol. 47, 655.
      Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
      Knowles, R.G., and Moncada, S. 1994. Biochem. J. 298, 249.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min at 37°C in a solution containing 50 mM HEPES, 5 µM oxyhemoglobin, 1 mM CaCl₂, 20 µl calmodulin, 0.1 mM NADPH, 50 µM arginine, 12 µM tetrahydrobiopterin, and 170 µM DTT, pH 7.5.
      FormLiquid
      FormulationIn 50 mM HEPES, 5 mM CHAPS, 100 µM DTT, 10% glycerol, pH 7.4.
      Applications
      Biological Information
      Specific Activity~2 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      482732 0

      Documentation

      Nitric Oxide Synthase, Constitutive, Bovine Endothelial, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      482732

      References

      Reference overview
      Busconi, L., and Michel, T. 1995. Mol. Pharmacol. 47, 655.
      Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
      Knowles, R.G., and Moncada, S. 1994. Biochem. J. 298, 249.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2008 RFH
      SynonymseNOS, NOS III, Endothelial Nitric Oxide Synthase
      DescriptionRecombinant bovine endothelial NOS expressed in S. frugiperda insect cells using a baculovirus expression system. eNOS undergoes several post-translational modifications, including myristoylation and palmitoylation, which causes it to become associated with membrane. Useful for studies involving NOS regulation, inhibitor screening, and kinetic analysis.

      Note: eNOS is an unstable enzyme. Keep the stock vial of the enzyme on ice at all times during the experiment. The enzyme should be added to the incubation medium just prior to the initiation of the experiment.
      FormLiquid
      FormulationIn 50 mM HEPES, 5 mM CHAPS, 100 µM DTT, 10% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Specific activity~2 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min at 37°C in a solution containing 50 mM HEPES, 5 µM oxyhemoglobin, 1 mM CaCl₂, 20 µl calmodulin, 0.1 mM NADPH, 50 µM arginine, 12 µM tetrahydrobiopterin, and 170 µM DTT, pH 7.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBusconi, L., and Michel, T. 1995. Mol. Pharmacol. 47, 655.
      Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
      Knowles, R.G., and Moncada, S. 1994. Biochem. J. 298, 249.