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482732 Nitric Oxide Synthase, Constitutive, Bovine Endothelial, Recombinant, S. frugiperda

Nitric Oxide Synthase, Constitutive, Bovine Endothelial, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: eNOS, NOS III, Endothelial Nitric Oxide Synthase

482732
  
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      Overview

      Description
      Overview

      This product has been discontinued.





      Recombinant bovine endothelial NOS expressed in S. frugiperda insect cells using a baculovirus expression system. eNOS undergoes several post-translational modifications, including myristoylation and palmitoylation, which causes the enzyme to become membrane-associated.
      Catalogue Number482732
      Brand Family Calbiochem®
      SynonymseNOS, NOS III, Endothelial Nitric Oxide Synthase
      References
      ReferencesBusconi, L., and Michel, T. 1995. Mol. Pharmacol. 47, 655.
      Hevel, J.M., and Marletta, M.A. 1994. Methods Enzymol. 233, 250.
      Knowles, R.G., and Moncada, S. 1994. Biochem. J. 298, 249.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min at 37°C in a solution containing 50 mM HEPES, 5 µM oxyhemoglobin, 1 mM CaCl₂, 20 µl calmodulin, 0.1 mM NADPH, 50 µM arginine, 12 µM tetrahydrobiopterin, and 170 µM DTT, pH 7.5.
      FormLiquid
      FormulationIn 50 mM HEPES, 5 mM CHAPS, 100 µM DTT, 10% glycerol, pH 7.4.
      Biological Information
      Specific Activity~2 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      482732 0