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411543 Sigma-AldrichMouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate

This Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG₁, Fc Fragment Specific.

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

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Overview

Replacement Information

Pricing & Availability

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Description
Catalogue Number	411543
Brand Family	Calbiochem®

References
References	Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Hamilton, R.G. 1987. Clin. Chem. 33, 1707. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.

Product Information
Form	Liquid
Formulation	In PBS, 1% BSA, pH 7.4.
Preservative	None
Quality Level	MQ100

Applications
Application References	Cross-Reactivity, Myeloma Proteins Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Letters 10, 223. Cross-Reactivity, Chimeric Antibodies Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Key Applications	Enzyme Immunoassay
Application Notes	Enzyme Immunoassay (1:500-1:2000)
Application Comments	Antibody should be titrated for optimal results in individual systems. Cross-reactivity by ELISA against human myeloma proteins: Human IgG₁: 100% Human IgG₂: ≤0.1% Human IgG₃: ≤0.2% Human IgG₄: ≤0.1% Human IgM: ≤0.1% Human IgA: ≤0.1% Human IgE: ≤0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG₁: 100% Human IgG₂: <0.02% Human IgG₃: <0.02% Human IgG₄: <0.02% Human IgM: <0.02% Human IgA: <0.01% Human IgE: <0.001%

Biological Information
Clone	HP6069
Host	Mouse
Isotype	IgG
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
411543-200UG	07790788050078

Documentation

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate MSDS

Title
Safety Data Sheet (SDS)

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate Certificates of Analysis

Title	Lot Number
411543	Enter Lot Number

References

Reference overview
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Hamilton, R.G. 1987. Clin. Chem. 33, 1707. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	06-August-2007 RFH
Application	Enzyme Immunoassay (1:500-1:2000)
Description	Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG₁ Fc fragment.
Background	The HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the Calbiochem^® technical monograph, The Human IgG Subclasses, is available free of charge.
Host	Mouse
Clone	HP6069
Isotype	IgG
Form	Liquid
Formulation	In PBS, 1% BSA, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	None
Comments	Antibody should be titrated for optimal results in individual systems. Cross-reactivity by ELISA against human myeloma proteins: Human IgG₁: 100% Human IgG₂: ≤0.1% Human IgG₃: ≤0.2% Human IgG₄: ≤0.1% Human IgM: ≤0.1% Human IgA: ≤0.1% Human IgE: ≤0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG₁: 100% Human IgG₂: <0.02% Human IgG₃: <0.02% Human IgG₄: <0.02% Human IgM: <0.02% Human IgA: <0.01% Human IgE: <0.001%
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Hamilton, R.G. 1987. Clin. Chem. 33, 1707. Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
Application references	Cross-Reactivity, Myeloma Proteins Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Letters 10, 223. Cross-Reactivity, Chimeric Antibodies Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.

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