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71863 Gateway® Nova pET-60-DEST™ Expression System

71863
  
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      Overview

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      This product has been discontinued.





      The Gateway® Nova pET-60-DEST™ vector is designed to create expression clones (pEXPR) that are fused to an N-terminal GST•Tag™ coding sequence and a thrombin cleavage site. The GST•Tag enables gentle purification of fusion proteins using GST•Bind® resin. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will also be fused to a C-terminal His•Tag® coding sequence. The presence of a "gentle elution" tag at each terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation and for recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.
      Catalogue Number71863
      Brand Family Novagen®
      References
      Product Information
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      Biological Information
      Physicochemical Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/38

      Irritating to eyes and skin.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Irritant
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71863 0