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70532 GST•Tag™ Assay Kit

Quantitative assay of GST•Tag fusion proteins

GST•Tag™ Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70532
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The GST•Tag™ Assay Kit is designed to perform quantitative colorimetric enzymatic assays of glutathione-S-transferase (GST) fusion proteins (Habig 1974). The kit is useful for the quantification of GST activity in crude samples or purified fractions. The suitability of this assay for crude samples allows expression conditions to be evaluated and rapidly optimized by comparing GST activity levels. The GST activity assay is simple to perform using the supplied 1-chloro-2,4-dinitrobenzene (CDNB) substrate. A sample is combined with CDNB substrate in the supplied reaction buffer and the absorbance of the reaction is monitored at 340 nm. The rate of change in A340 is proportionate to the amount of GST activity present in the sample. The assay has sufficient sensitivity to detect as little as eight pmol of functional GST, which corresponds to approximately 250 ng of unfused GST.
      Catalogue Number70532
      Brand Family Novagen®
      References
      ReferencesHabig, W.H., et al. 1974. J. Biol. Chem. 249, 7130.
      Product Information
      •2 x 5 ml10X GST•Tag Assay Buffer
      •1.2 ml100 mM CDNB
      •1.2 ml100 mM Reduced Glutathione
      •50 µgGST•Tag Standard
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 11-23/24/25-33-36/37/38-50/53

      Highly flammable.
      Toxic by inhalation, in contact with skin and if swallowed.
      Danger of cumulative effects.
      Irritating to eyes, respiratory system and skin.
      Very toxic to aquatic organisms; may cause long-term adverse effects in the aquatic environment.
      S PhraseS: 7-16-26-36/37-45-28-60-61

      Keep container tightly closed.
      Keep away from sources of ignition - No Smoking.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing and gloves.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

      This material and its container must be disposed of as hazardous waste.
      Avoid release to the environment. Refer to special instructions/safety data sheet.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70532 0

      Documentation

      GST•Tag™ Assay Kit Certificates of Analysis

      TitleLot Number
      70532

      References

      Reference overview
      Habig, W.H., et al. 1974. J. Biol. Chem. 249, 7130.

      Citations

      Title
    • John K. Mensah, et al. (2004) Functional analysis of a mutation in PAX9 associated with familial tooth agenesis in humans. Journal of Biological Chemistry 279, 5924-5933.
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      User Protocols

      Title
      TB236 GST•Tag™ Assay Kit